TAMRA CPG 1000, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17760 | 100 mg |
$95.00
|
in stock | |
| 47760 | 1 g |
$469.00
|
in stock |
6-TAMRA CPG (glycerol) is controlled pore glass (CPG) with the fluorescent dye 6-carboxytetramethylrhodamine (6-TAMRA) covalently attached via a glycerol linker. This support is designed for automated solid-phase oligonucleotide synthesis using the phosphoramidite method, allowing the introduction of a fluorescent label at the 3′-end without additional post-synthetic labeling steps.
The 1000 Å pore size is optimal for synthesizing long sequences — up to 100 nucleotides. 6-TAMRA is a orange-red fluorescent dye with absorption and emission maxima at 541 nm and 567 nm, respectively, and is widely used in molecular diagnostics. TAMRA-labeled oligonucleotides obtained with this reagent are employed in methods such as real-time PCR, fluorescence in situ hybridization (FISH), FRET, and surface-enhanced Raman spectroscopy (SERS).
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General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30-40 |
| Coupling conditions: | standard time depending on the first monomer |
| Cleavage conditions: | tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature. |
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