dsGreen Nucleic Acid Gel Staining Solution, 10000×

PCR and qPCR
Cat. # Quantity Price Lead time
A0010 50 uL –   in stock
10010 0.5 mL $135.00 in stock
20010 1 mL $229.00 in stock
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dsGreen is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

FeatureEthidium BromidedsGreen
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band (dsDNA)
100 ng / band (RNA)		 0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
Health hazard High Low

 

1% agarose gels with variable amount of plasmid stained by either dsGreen, or ethidium bromide. Plasmid quantities are in nanograms.
1% agarose gels with variable amount of plasmid stained by either dsGreen, or ethidium bromide. Plasmid quantities are in nanograms.
dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
dsGreen I structure
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Excitation and emission spectra of dsDNA complex with dsGreen

Excitation and emission spectra of dsDNA complex with dsGreen

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General properties

Appearance: orange solution
Quality control: NMR 1H, HPLC-MS (95%), functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8

Kit

Storage time in months: 12

Product citations

  1. Chetverikov, P. E.; Craemer, C.; Gankevich, V. D.; Kremenetskaya, M. V.; Kuzmin, I. V.; Zhuk, A. S. Endoparasitic Gall Mites: Two New Novophytoptus Species (Eriophyoidea, Phytoptidae) from Southern African Sedges (Cyperaceae, Carex) and New Hypotheses on the Phylogeny of Novophytoptines. Diversity, 2023, 15(3), 416;. doi: 10.3390/d15030416
  2. Gmoshinskiy, V. I.; Novozhilov, Yu. K.; Prikhodko, I. S.; Bortnikov, F. M.; Shchepin, O. N.; Schnittler, M. Morphology and phylogeny of Diderma aurantiacum (Myxomycetes) — a new species for Russia from the Far East. Novosti sistematiki nizshikh rastenii, 2023, 57(1), 27–42. doi: 10.31111/nsnr/2023.57.1.27
  3. Chetverikov, Ph.E.; Desnitskiy, A.G.; Klimov, P.B.; Ozman- Sullivan, S.K.; Romanovich, A.E.; Sukhareva, S.I. Deuterogyny and the Association of Two Vagrant Eriophyoid Mites (Acariformes, Eriophyoidea) with the Host-Plant Generative Organs of Two Broad-Leaved Trees in North-West Russia. Zoological Studies, 2023, 62, 35. doi: 10.6620/ZS.2023.62-35
  4. Abil, Z.; Restrepo Sierra, A. M.; Danelon, C. Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles. ACS Synth Biol, 2023, 12(4), 1187–1203. doi: 10.1021/acssynbio.2c00668
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