dsGreen Gel Staining Solution, 10000×

PCR and qPCR
Cat. # Quantity Price Lead time
A0010 50 uL $29.00 in stock
10010 0.5 mL $100.00 in stock
20010 1 mL $180.00 in stock
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dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

FeatureEthidium BromidedsGreen
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band (dsDNA)
100 ng / band (RNA)		 0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
Health hazard High Low

Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
1% agarose gels with variable amount of plasmid stained by either dsGreen, or ethidium bromide. Plasmid quantities are in nanograms.
1% agarose gels with variable amount of plasmid stained by either dsGreen, or ethidium bromide. Plasmid quantities are in nanograms.
dsGreen I structure
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Recommended protocol

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Pico488 dsDNA quantification reagent, 200x solution in DMSO

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DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.

ROX reference dye for qPCR

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General properties

Appearance: orange solution
Quality control: NMR 1H, HPLC-MS (95%), functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 454
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8

Product citations

  1. Komarova, N.; Andrianova, M.; Glukhov, S.; Kuznetsov, A. Selection, Characterization, and Application of {ssDNA} Aptamer against Furaneol. Molecules, 2018, 23(12), 3159. doi: 10.3390/molecules23123159
  2. Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, 2017, 220(2), 251–262. doi: 10.1111/apha.12820
  3. Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi: 10.1002/jcp.25682
  4. Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi: 10.3389/fmicb.2016.00773
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SYBR® is a registered trademark of Molecular Probes.
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