Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 33030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 43030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A3030 | 1 mg | $110.00 | in stock | |
| B3030 | 5 mg | $210.00 | in stock | |
| C3030 | 10 mg | $310.00 | in stock | |
| D3030 | 25 mg |
$410.00
|
in stock | |
| E3030 | 50 mg |
$695.00
|
in stock | |
| F3030 | 100 mg |
$1190.00
|
in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.
Cyanine5 absorbance and emission spectra
Recommended protocol
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Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of Click chemistry.Click chemistry buffer, 1.5x
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Azide of green-yellow emitting HEX dye is used for conjugation with oligonucleotides via [3+2]-dipolar cycloaddition.General properties
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| CAS number: | 1267539-32-1 (chloride) |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Thauvin, C.; Maudens, P.; Allémann, E. Microwave-assisted synthesis of self-assembling bi-functionalizable amphiphilic diblock copolymers. Journal of Drug Delivery Science and Technology, 2021, 61, 102255. doi: 10.1016/j.jddst.2020.102255
- Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019
- Kim, S.M.; Park, J.; Kim, M.S.; Song, H.; Jo, A.; Park, H.; Kim, T.S.; Choi, S.H.; Park, S.B. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infectious Diseases, 2020, 6(11), 3076–3082. doi: 10.1021/acsinfecdis.0c00587
- von Döhren, J.; Kuhl, S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. Journal of Biological Methods, 2020, 7(2), e132. doi: 10.14440/jbm.2020.320
Cy® is a trademark of Cytiva.
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