Protein Biotinylation Kit

Protein labeling Biotinylation
Cat. # Quantity Price Lead time
BB21-1rxn
/ for 100 ug IgG
1 rxn
$156.00
1 days
BB21-10rxn
/ for 10× 100 ug IgG
10 rxn
$389.00
21 days
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Protein Biotinylation Kit is a ready-to-use kit for covalent labeling of purified proteins, peptides, and antibodies with biotin using activated NHS-ester biotin. The kit provides fast, reproducible, and gentle biotinylation of primary amino groups (the ε-amino groups of lysine residues and N-terminal amino groups) without significantly affecting the protein’s biological activity.

The optimized protocol enables biotinylation in a short time and allows control over the degree of labeling by adjusting the reagent/protein ratio. The resulting biotinylated conjugates are fully compatible with streptavidin-biotin systems and with standard detection and affinity-immobilization methods.

Key Features

  • One-step biotinylation of primary amino groups using NHS-biotin
  • Fast reaction at physiological and slightly alkaline pH
  • Suitable for most purified peptides, proteins, and antibodies
  • Does not require prior protein modification
  • Preserves native protein properties due to mild reaction conditions
  • Compatible with ELISA, Western blotting, enzyme immunoassay, immunochemistry, flow cytometry, and affinity methods

Applications

  • Preparation of biotinylated antibodies and antigens
  • Streptavidin/avidin-based immunoassays
  • Affinity immobilization of proteins
  • Cellular and biochemical research
  • Development of diagnostic reagents

We also produce a wide range of fluorescently labeled streptavidins for immunochemistry.

Protein Biotinylation Kit, BB21-1rxn

Ready-to-use kit manual

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General properties

Storage conditions: Store at temperature between 4 °C and 20 °C. Do not freeze! Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Kit

Storage time in months: 12
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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