Perylene dU phosphoramidite

Cat. # Quantity Price Lead time
14460 100 mg $150 in stock
24460 250 mg $320 in stock
44460 1 g $880 in stock
64460 5 g please inquire 21 days
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Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.

With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis. Perylene is attached to the 5′ position of deoxyuridine (dU) through a triple bond and the fluorophore is electronically coupled to the deoxyuridine base. This coupling of dU and perylene makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent is acetonitrile.

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General properties

Appearance: orange solid
Molecular weight: 1005.1
CAS number: 908117-78-2
Molecular formula: C61H57N4O8P
Solubility: good in dichloromethane and acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 435; 408; 252
ε, L⋅mol−1⋅cm−1: 36000
Emission maximum, nm: 439; 467
Fluorescence quantum yield: 1.0

Oligo synthesis details

Diluent: acetonitrile
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