BDP FL maleimide

Cat. # Quantity Price Lead time
11480 1 mg $110.00 in stock
21480 5 mg $210.00 in stock
41480 25 mg $410.00 in stock
51480 50 mg $695.00 in stock
61480 100 mg $1190.00 in stock

Thiol reactive BDP FL maleimide is a reactive dye for protein labeling, which has identical structure with BODIPY® FL maleimide.

BDP FL is a borondipyrromethene dye which has absorption and fluorescence spectra similar to fluorescein (FAM). However, this dye exhibits very high photostability. It is non-charged, and has low molecular weight. Its brightness is similar to fluorescein, R110 and xanthene dye derivatives like Alexa Fluor® 488.

This fluorophore is ideal for fluorescent microscopy and many other applications. The fluorophore can substitute fluorescein for almost any application, and it is compatible with any FAM-capable fluorescent instrumentation.

Absorption and emission spectra of BDP FL

Absorption and emission spectra of BDP FL

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General properties

Appearance: orange solid
Mass spec M+ increment: 414.1
Molecular weight: 414.21
Molecular formula: C20H21BF2N4O3
Solubility: Good in organic solvents (DMF, DMSO), limited in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

Product citations

  1. São Pedro, M.N.; Azevedo, A.M.; Aires-Barros, M.R.; Soares, R.R.G. Minimizing the influence of fluorescent tags on IgG partition in PEG-salt aqueous two-phase systems for rapid screening applications. Biotechnology Journal, in press. doi: 10.1002/biot.201800640
  2. Doh, J.K.; White, J.D.; Zane, H.K.; Chang, Y.H.; López, C.S.; Enns, C.A.; Beatty, K.E. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115, 12961–12966. doi: 10.1073/pnas.1808626115
  3. Buecheler, J.W.; Winzer, M.; Tonillo, J.; Weber, C.A.; Gieseler, H. Impact of Payload Hydrophobicity on Stability of Antibody-Drug-Conjugates. Molecular Pharmaceutics, 2018, 15(7), 2656–2664. doi: 10.1021/acs.molpharmaceut.8b00177
  4. Mardirossian, M.; Pérébaskine, N.; Benincasa, M.; Gambato, S.; Hofmann, S.; Huter, P.; Müller, C.; Hilpert, K.; Innis, C.A.; Tossi, A.; Wilson, D.N. The Dolphin Proline-Rich Antimicrobial Peptide Tur1A Inhibits Protein Synthesis by Targeting the Bacterial Ribosome. Cell Chemical Biology, 2018, 25(5), 530–539.e7. doi: 10.1016/j.chembiol.2018.02.004
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