AF488 NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
11820 | 1 mg | $110.00 | in stock | |
21820 | 5 mg | $290.00 | in stock | |
31820 | 10 mg |
$470.00
|
5 days | |
41820 | 25 mg |
$690.00
|
in stock | |
51820 | 50 mg |
$1270.00
|
in stock | |
61820 | 100 mg |
$1990.00
|
in stock |
AF488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.
AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.
This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
Absorption and emission spectra of AF488

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Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.General properties
Appearance: | dark orange solid |
Molecular weight: | 732.74 |
Molecular formula: | C31H32N4O13S2 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid) |
Storage conditions: | Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
ε, L⋅mol−1⋅cm−1: | 71800 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
CF260: | 0.16 |
CF280: | 0.10 |
Product citations
- Eliseev, I.E.; Ukrainskaya, V.M.; Yudenko, A.N.; Mikushina, A.D.; Shmakov, S.V.; Afremova, A.I.; Ekimova, V.M.; Vronskaia, A.A.; Knyazev, N.A.; Shamova, O.V. Targeting ErbB3 Receptor in Cancer with Inhibitory Antibodies from Llama. Biomedicines, 2021, 9(9), 1106. doi: 10.3390/biomedicines9091106
- Huang, D.; Yue, F.; Qiu, J.; Deng, M.; Kuang, S. Polymeric nanoparticles functionalized with muscle-homing peptides for targeted delivery of phosphatase and tensin homolog inhibitor to skeletal muscle. Acta Biomaterialia, 2020, 118, 196–206. doi: 10.1016/j.actbio.2020.10.009
