PreciseGreen dsDNA Quantification Reagent, 200×

PCR and qPCR Nucleic acid quantification
Cat. # Quantity Price Lead time
12010 100 uL –   in stock
42010 1 mL $360 in stock
62010 25 mL please inquire in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

PreciseGreen is a DNA-binding benzothiazole dye. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double-stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and dedicated DNA measurement fluorometers.

The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit, including quantification dye, is also available.

PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen fluorescence emission vs DNA concentration
Fluorescence of the PreciseGreen Quantitation Reagent upon binding with dsDNA, ssDNA and RNA
Structure of PreciseGreen
Download in
ChemDraw format

PreciseGreen excitation and emission spectra

PreciseGreen excitation and emission spectra

Recommended protocol

Calculator

Customers also purchased with this product

Cyanine5.5 DBCO

Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for copper-free click chemistry.
Add this product to your cart and
get free express delivery

Cyanine7.5 carboxylic acid

Cyanine7.5 - unactivated NIR cyanine dye carboxylic acid.
Add this product to your cart and
get free express delivery

Azide-PEG3-amine

A bifunctional linker with azide and amine groups flanking PEG3 (triethylene glycol)

General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
Emission maximum, nm: 526

Product citations

  1. Vellani, V.; Cuccaro, A.; Oliva, M.; Pretti, C.; Renzi, M. Assessing combined effects of long-term exposure to copper and marine heatwaves on the reef-forming serpulid Ficopomatus enigmaticus through a biomarker approach. Marine Pollution Bulletin, 2024, 201, 116269. doi: 10.1016/j.marpolbul.2024.116269
  2. Hernández-Rollán, C.; Ehrmann, A.K.; Vlassis, A.; Kandasamy, V.; Nørholm, M.H.H. Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR. BMC Biotechnology, 2024, 24, 17. doi: 10.1186/s12896-024-00844-7
  3. Kang, Z.; Fu, P.; Ma, H.; Li, T.; Lu, K.; Liu, J.; Ginjala, V.; Romanienko, P.; Feng, Z.; Guan, M.; Ganesan, S.; Xia, B. Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy. bioRxiv, 2023, preprint. doi: 10.1101/2023.10.03.560719
  4. Hiroki, H.; Ishii, Y.; Piao, J.; Namikawa, Y.; Masutani, M.; Honda, H.; Akahane, K.; Inukai, T.; Morio, T.; Takagi, M. Targeting Poly(ADP)Ribose Polymerase in BCR/ABL1-Positive Cells. Scientific Reports, 2023, 13(1), 7588. doi: 10.1038/s41598-023-33852-2
Show more (16)
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.