DusQ 3 CPG 500
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1618-100mg | 100 mg | – | in stock | |
1618-1g | 1 g | $990 | in stock |
DusQ 3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.
The dye can be attached to 3'-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.
Absorption spectrum of DusQ 3
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TFA-amino modifier CPG 500
TFA-amino modifier CPG for oligo synthesis. 500 Å, high load controlled pore glass.JOE phosphoramidite, 6-isomer
JOE dye phosphoramidite for synthesis of labeled oligonucleotides. JOE absorption and emission maxima are between maxima of FAM and TAMRA/ROX dyes.
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General properties
Appearance: | dark blue beads |
Quality control: | 1H NMR of bound reagent, coupling testing with HPLC-MS |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 657 |
ε, L⋅mol−1⋅cm−1: | 45500 |
Oligo synthesis details
Pore size, Å: | 500 |
Typical loading, umol/g: | 50-80 |
Coupling conditions: | standard, ultramild phosphoramidites |
Cleavage conditions: | 2 h / rt, 30% aq. ammonia (ultramild protective groups) |
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