Cyanine3 maleimide

Cat. # Quantity Price Lead time
11080 1 mg $125 in stock
21080 5 mg $260 in stock
41080 25 mg $510 in stock
51080 50 mg $895 in stock
61080 100 mg $1490 in stock
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Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

Cy3 absorbance and emission spectra

Cy3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 666.56
Molecular formula: C36H43N4O3BF4
Solubility: well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Chadda, R.; Lee, T.; Mahoney-Kruszka, R.; Kelley, E. G.; Bernhardt, N.; Sandal, P.; Robertson, J. L. A Thermodynamic Analysis of CLC Transporter Dimerization in Lipid Bilayers. Proceedings of the National Academy of Sciences, 2023, 120(41), e2305100120. doi: 10.1073/pnas.2305100120
  2. Song, Q.-X.; Liu, N.-N.; Liu, Z.-X.; Zhang, Y.-Z.; Rety, S.; Hou, X.-M.; Xi, X.-G. Nonstructural N- and C-Tails of Dbp2 Confer the Protein Full Helicase Activities. Journal of Biological Chemistry, 2023, 299(5), 104592. doi: 10.1016/j.jbc.2023.104592
  3. Naeimipour, S.; Boroojeni, F. R.; Lifwergren, P.; Selegård, R.; Aili, D. Multimodal andDynamic Cross-Linking of Modular Thiolated Alginate-Based Bioinks. Materials Today Advances, 2023, 19, 100415. doi: 10.1016/j.mtadv.2023.100415
  4. Binda, O.; Juillard, F.; Ducassou, J.N.; Kleijwegt, C.; Paris, G.; Didillon, A.; Baklouti, F.; Corpet, A.; Couté, Y.; Côté, J.; Lomonte, P. SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Science Alliance, 2023, 6(1), e202201429. doi: 10.26508/lsa.202201429
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