AF 594 biocytin

Neuroscience Microscopy
Cat. # Quantity Price Lead time
3703-50ug 50 ug $39 21 days
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

AF 594 biocytin is a cell-impermeant polar tracer. It consists of AF 594 dye, biotin, and aldehyde-fixable primary amine.

AF 594 is a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm). Biotin allows the detection of the tracer with enzyme-containing streptavidin and subsequent amplification.

Polar tracers are water-soluble and can be introduced into cells by iontophoresis, patch clamping, osmotic lysis of pinocytic vesicles, and other comparable methods. AF 594 biocytin can be used to examine plasma membrane permeability, pinocytosis, transport through gap junctions, and cell-cell or cell-liposome fusion. It can also be used as a retrograde and anterograde neuronal tracer in live tissue.

Stock solution can be prepared in DMSO or DMF before use.

Absorption and emission spectra of AF 594

Absorption and emission spectra of AF 594

Customers also purchased with this product

6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)

A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.

Biocytin hydrochloride

Lysine derivative of biotin for conjugation with avidin or streptavidin, neuroanatomical tracer.

General properties

Appearance: blue solid
Molecular weight: 1279.66
Molecular formula: C63H90N8O14S3
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 586
ε, L⋅mol−1⋅cm−1: 105000
Emission maximum, nm: 613
Fluorescence quantum yield: 0.77
CF260: 0.28
CF280: 0.51
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.