DusQ® 2 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12960 | 100 mg | – | in stock | |
| 32960 | 500 mg |
$530
|
in stock | |
| 42960 | 1 g |
$990
|
in stock | |
| 52960 | 5 g | please inquire | in stock |
This support is intended for automated synthesis of oligonucleotides with 3′-terminal DusQ® 2 quencher. Pore size of 500 Å is recommended for the synthesis of oligonucleotides of up to 50 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar® 670, Cyanine5.5, and Quasar® 705.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
Absorption spectrum of DusQ 2
Customers also purchased with this product
FAM phosphoramidite, 6-isomer
FAM phosphoramidite 6-isomer, is a widely used reagent in nucleic acid chemistry for labeling oligonucleotides. It is derived from the single pure isomer of 6-carboxyfluorescein and separated from amidite linkage via C6 aminohexanol spacer.TBTA ligand
TBTA is one of the most widely used water-insoluble ligands for copper-catalyzed azide-alkyne cycloadditions (CuAAC).Cyanine5 azide
Cyanine5 azide is a reagent for click chemistry labeling with Cyanine5, a popular fluorescent dye.
Add this product to your cart andget free express delivery
General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
Your cart is empty
$ 
