AF 488 azide

Cat. # Quantity Price Lead time
S1830 250 ug –   in stock
11830 1 mg $125 in stock
21830 5 mg $325 in stock
41830 25 mg $950 in stock
51830 50 mg $1625 in stock
61830 100 mg $2090 in stock
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AF 488 is a fluorescent dye. AF 488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF 488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.

AF 488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: orange solid
Molecular weight: 692.76
CAS number: 1679326-36-3 (with azidohexyl group)
Molecular formula: C24H18K2N6O10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10

Product citations

  1. Baleva, M.V.; Piunova, U.; Chicherin, I.; Vasilev, R.; Levitskii, S.; Kamenski, P. Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells. International Journal of Molecular Sciences, 2024, 25(1), 93. doi: 10.3390/ijms25010093
  2. Ivanova, A.; Gruzova, O.; Ermolaeva, E.; Astakhova, O.; Itaman, S.; Enikolopov, G.; Lazutkin, A. Synthetic Thymidine Analog Labeling without Misconceptions. Cells, 2022, 11(12), 1888. doi: 10.3390/cells11121888
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