PKH800 Cell Membrane Labeling Kit

Cell proliferation Cell membrane Microscopy Flow cytometry Near infrared (NIR) imaging
Cat. # Quantity Price Lead time
27201
100 uL dye, 5x buffer
$225 1 days
17201
100 uL dye, 1x buffer
$290 1 days
47201
500 uL dye, 5x buffer
$1125 1 days
37201
500 uL dye, 1x buffer
$1230 21 days
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The ready-to-use kit contains all necessary reagents to label cell membranes with PKH800 dye for evaluating cell migration or proliferation.

The kit contains PKH800 cyanine dye with lipophilic groups, which allow rapid and non-covalent incorporation into cell membranes of almost any cells without influence on cell receptors or transmembrane proteins. The cells preserve their biological properties and ability to proliferate, which makes PKH800 an excellent fluorophore for studying plant and animal cells in vivo and in vitro. It can also be used for studying membrane vesicles.

PKH800 is a near-infrared fluorescent dye with an emission maximum at 797 nm. It can be used with other dyes in multicolor assays and, especially, in applications requiring low sample background fluorescence. PKH800 is rather stable, and PKH800-labeled cells can be studied by microscopy or flow cytometry within several days after staining.

The number of measurements using the kit can vary depending on the cell count in the sample:

  • The kit containing 100 µL of PHK800 allows analyzing 25 samples of 20 million cells each, using the final PKH800 concentration of 2×10-6 M.
  • The kit containing 500 µL of PHK800 allows analyzing 125 samples of 20 million cells each, using the final PKH800 concentration of 2×10-6 M.

We recommend choosing the necessary cell count and concentration depending on the cell type and study objectives.

PKH800 cell membrane labeling kit, 27201
PKH800 cell membrane labeling kit, 27201

Product in action

Foci of endometriosis detected using PKH800 cell membrane labeling kit

Absorption and emission spectra of PKH800

Absorption and emission spectra of PKH800

Ready-to-use kit manual

Calculator

General properties

Storage conditions: Store at 4 °С. Warm to RT before use.
Fragment weight:
MSDS: Download

Spectral properties

Excitation/absorption maximum, nm: 791
ε, L⋅mol−1⋅cm−1: 270500
Emission maximum, nm: 809

Kit

Storage time in months: 12
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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