PKH2 cell membrane labeling kit

Cat. # Quantity Price Lead time
100 uL dye, 5x buffer
$225 1 days
100 uL dye, 1x buffer
$290 1 days
500 uL dye, 5x buffer
$1125 1 days
500 uL dye, 1x buffer
$1230 21 days
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The ready-to-use kit contains all necessary reagents to label cell membranes with PKH2 dye for evaluating cell migration or proliferation.

The kit contains PKH2 cyanine dye with lipophilic groups, which allow rapid and non-covalent incorporation into cell membranes of almost any cells without the influence on cell receptors or transmembrane proteins. The cells preserve their biological properties and ability to proliferate, which makes PKH2 an excellent fluorophore for studying plant and animal cells in vivo and in vitro. It can also be used for studying membrane vesicles.

PKH2 is a green fluorescent dye with an emission maximum at 509 nm. It can be used with other dyes in multicolor assays. PKH2 is less stable than PKH26, and labeled cells can be studied by microscopy or flow cytometry within 10-11 days after staining.

The number of measurements using the kit can vary depending on the cell count in the sample:

  • The kit containing 100 µL of PHK2 allows analyzing 25 samples of 20 million cells each, using the final PKH2 concentration of 2×10-6 M.
  • The kit containing 500 µL of PHK2 allows analyzing 125 samples of 20 million cells each, using the final PKH2 concentration of 2×10-6 M.

We recommend choosing the necessary cell count and concentration depending on the cell type and study objectives.

Absorption and emission spectra of PKH2

Absorption and emission spectra of PKH2

Ready-to-use kit manual


General properties

Storage conditions: Store at 4 °С. Warm to RT before use.
MSDS: Download

Spectral properties

Excitation/absorption maximum, nm: 487
ε, L⋅mol−1⋅cm−1: 128000
Emission maximum, nm: 502
Fluorescence quantum yield: 0.21


Storage time in months: 12
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