Annexin V-AF 647 Apoptosis Detection Kit, with Propidium Iodide

Cell viability and apoptosis Microscopy Flow cytometry
Cat. # Quantity Price Lead time
14172
10 assays
–   1 days
24172
50 assays
$148 1 days
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Annexin V (or Annexin A5) is a member of the phospholipid-binding annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine (PS) in a calcium-dependent manner. The method was first reported by Koopman et al. (1994) [1].

In healthy cells, PS is normally retained in the inner leaflet of the plasma membrane. The early stage of apoptosis is accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of PS at the cell's surface. In conjunction with vital dyes such as propidium iodide (PI), fluorochrome-labeled Annexin V can distinguish between healthy (Annexin−PI−), apoptotic (Annexin+PI−), and necrotic (Annexin+PI+) cells [2].

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The ready-to-use kit contains all necessary reagents to label apoptotic cells with Annexin V-AF 647 conjugate and PI.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes it indispensable for labeling cells with high autofluorescence. The absorption and emission maxima of AF 647 are at 655 and 680 nm, respectively.

PI is a membrane-impermeable DNA stain, making it useful for differentiating necrotic, apoptotic, and healthy cells based on membrane integrity. After DNA binding, the dye emits in the orange-red channel. Its absorption max. is at 535 nm, the emission max. is at 617 nm.

[1] Koopman G. et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994. 84(5). P.1415-1420.

[2] Martin S.J. et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 1995. 182(5). P.1545-1556.

Annexin V-AF 647 Apoptosis Detection Kit, with Propidium Iodide, 24172
Annexin V-AF 647 Apoptosis Detection Kit, with Propidium Iodide, 24172

Absorption and emission spectra of AF 647 and Propidium Iodide

Absorption and emission spectra of AF 647 and Propidium Iodide

Ready-to-use kit manual

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Annexin V-AF 488 Apoptosis Detection Kit, with Propidium Iodide

The ready-to-use kit to label apoptotic and necrotic cells with Annexin V-AF 488 conjugate and propidium iodide.

Propidium Iodide

Propidium iodide is a membrane-impermeable intercalating DNA dye for staining nuclei of death cells.

General properties

Storage conditions: Transportation: at room temperature for 1 week. Store at -20°С.
MSDS: Download

Kit

Storage time in months: 9
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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