Alkyne phosphoramidite, 5'-terminal
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
|
22260
/ in 6 mL septum
|
250 mg |
$110.00
|
in stock | |
|
32260
/ in 6 mL septum
|
500 mg |
$199.00
|
in stock | |
|
42260
/ in 6 mL septum
|
1 g |
$350.00
|
in stock | |
|
62260
/ in 20 mL septum
|
5 g |
$1290.00
|
in stock | |
| 82260 | 10 g |
please inquire
|
in stock |
Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for click chemistry.
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life.
Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5'-terminal DMT group, no 5'-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.
Oligonucleotides with this modification are ideal for the use in click chemistry (see our recommended protocol).
Customers also purchased with this product
Far red / near infrared fluorescent Cyanine5.5 dye derivative for click chemistry.…
Water soluble sulfo-Cyanine5 fluorescent dye azide for click chemistry.…
Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamin…
Pyrene phosphoramidite with pyrene attached to deoxyuridine.…
Bright and photostable azide AF 647 for labeling alkyne-containing biomolecules and fluore…
Cy7 amine is a near infrared dye with free amine group for the conjugation with activated …
TET is a green-fluorescent fluorescein derivate with spectral properties similar to R6G, J…
Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimeth…
Lumiprobe® Reticulocyte Stain is a ready-to-use Thiazole Orange solution for determining a…
Phosphoramidite modifier for synthesizing oligonucleotides containing alkyne modification …
Biotin alkyne is a reactive affinity label which can be attached to biomolecules via click…
General properties
| Appearance: | colorless solid |
| Molecular weight: | 409.50 |
| CAS number: | 1417539-32-2 |
| Molecular formula: | C21H36N3O3P |
| IUPAC name: | trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine |
| Solubility: | good in acetonitrile and dichloromethane |
| Quality control: | NMR 1H (95%) and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | Standard coupling, identical to normal nucleobases. |
| Cleavage conditions: | standard deprotection |
| Deprotection conditions: | No deprotection required. Compatible with standard deprotection reagents. |
Product citations
- Gulyak, E. L.; Brylev, V. A.; Zhitlov, M. Y.; Komarova, O. A.; Ustinov, A. V.; Sapozhnikova, K. A.; Alferova, V. A.; Korshun, V. A.; Gvozdev, D. A. Indocarbocyanine–Indodicarbocyanine (sCy3–sCy5) Absorptive Interactions in Conjugates and DNA Duplexes. Molecules, 2025, 30(1), 57. doi: 10.3390/molecules30010057
- Brylev, V. A.; Ryabukhina, E. V.; Nazarova, E. V.; Samoylenkova, N. S.; Gulyak, E. L.; Sapozhnikova, K. A.; Dzarieva, F. M.; Ustinov, A. V.; Pronin, I. N.; Usachev, D. Y.; Kopylov, A. M.; Golovin, A. V.; Pavlova, G. V.; Ryazantsev, D. Y.; Korshun, V. A. Towards Aptamer-Targeted Drug Delivery to Brain Tumors: The Synthesis of Ramified Conjugates of an EGFR-Specific Aptamer with MMAE on a Cathepsin B-Cleavable Linker. Pharmaceutics, 2025, 16(11), 1434. doi: 10.3390/pharmaceutics16111434
- Farzan, V.M.; Kvach, M.V.; Aparin, I.O.; Kireev, D.E.; Prikazchikova, T.A.; Ustinov, A.V.; Shmanai, V.V.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA. Talanta, 2019, 194, 226–232. doi: 10.1016/j.talanta.2018.10.043
- Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/8
The count of items is incorrect.
Your cart is empty
$ 
