AF568 alkyne, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A48B0 | 1 mg | $110.00 | in stock | |
| B48B0 | 5 mg | $290.00 | in stock | |
| C48B0 | 10 mg |
$470.00
|
in stock | |
| D48B0 | 25 mg |
$690.00
|
in stock | |
| E48B0 | 50 mg |
$1270.00
|
in stock | |
| F48B0 | 100 mg |
$1990.00
|
in stock |
The excitation maximum of AF568 lies at 572 nm and its emission maximum is at 598 nm. AF568 has excellent photochemical stability and brightness.
AF568 alkyne derivate is an effective tool for labeling purposes. It is easily detected in the ROX channel with a high signal-to-noise ratio in different biological samples. Besides, AF568 alkyne is water soluble and insensitive to pH changes between pH 4 and pH 10. Mild reaction conditions are suitable for most biomolecules, cells, and tissues.
Bioconjugation with AF568 alkyne presents a powerful technique for the production of fluorescently labeled biomolecules. AF568 alkyne is recommended for visualization procedures, including fluorescence microscopy, flow cytometry, and other applications where label brightness and photostability are required.
Absorption and emission spectra of AF568
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EdU (5-ethynyl-2'-deoxyuridine)
EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.AF568 NHS ester
AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.AmdU (5-azidomethyl-2'-deoxyuridine)
Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.General properties
| Appearance: | violet powder |
| Mass spec M+ increment: | 731.2 |
| Molecular weight: | 807.97 |
| Molecular formula: | C36H31N3K2O10S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 572 |
| ε, L⋅mol−1⋅cm−1: | 94238 |
| Emission maximum, nm: | 598 |
| Fluorescence quantum yield: | 0.912 |
| CF260: | 0.4 |
| CF280: | 0.32 |
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