AF 488 TFP ester

AF 488 Protein labeling TFP esters
Cat. # Quantity Price Lead time
118T0 1 mg $165 in stock
218T0 5 mg $510 in stock
418T0 25 mg $1650 in stock
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AF 488 is a bright and photostable dye well suited for labeling sensitive proteins and antibodies due to its hydrophilicity. AF 488 is a sulfonated derivative of Rhodamine 110 (R110), and, like other rhodamines, AF 488 is represented by two (5- and 6-) isomers. Both isomers have almost identical photophysical properties but require their separation since using a mixture of isomers leads to the «doubling» of the peaks of the labeled products during HPLC and electrophoretic separation. This product contains isomerically pure 5-AF 488.

Tetrafluorophenyl (TFP) esters belong to a group of activated esters widely used to conjugate fluorophores to the primary and secondary amines of biomolecules. Like hydroxysuccinimide (NHS) esters, they form a strong amide bond between the dye and the compound being modified; however, TFP esters are more stable at the basic pH values typically associated with reactions with amines and are also less susceptible to spontaneous hydrolysis.

AF 488 TFP ester, 25 mg
AF 488 TFP ester, 25 mg
AF 488 TFP ester, 25 mg
Structure of AF 488 TFP ester
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Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: red crystals
Molecular weight: 681.53
CAS number: 2133404-55-2
Molecular formula: C27H13F4N2O11S2-
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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