AF 430 azide

Azides AF 430 Click chemistry

Cat. #	Quantity	Price	Lead time
A0830	1 mg	$125	in stock
B0830	5 mg	$325	in stock
D0830	25 mg	$950	in stock
E0830	50 mg	$1625	in stock
F0830	100 mg	$2090	in stock

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AF 430 is a fluorescent dye. It can be excited by 405 nm violet laser or 445 nm laser paired, for example, with 510/80 nm bandpass filter.

Azide moiety allows quick and effective labeling and detection of terminal alkynes via a copper-catalyzed click reaction (CuAAC) or of strained cyclooctynes via a copper-free click chemistry reaction (SPAAC). Mild reaction conditions are suitable for most biomolecules, cells, and tissues. The product is water soluble and insensitive to pH changes between pH 4 and pH 10.

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Absorption and emission spectra of AF 430

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	yellow solid
Molecular weight:	623.69
Molecular formula:	C₂₅H₂₉N₅F₃KO₆S
Solubility:	good in water, DMF, DMSO
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	430
ε, L⋅mol⁻¹⋅cm⁻¹:	15955
Emission maximum, nm:	542
Fluorescence quantum yield:	0.23

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.