LumiSpin® BB, DNA Isolation Spin Kit for Blood and Buccal Swab

DNA isolation
Cat. # Quantity Price Lead time
21553
50 minipreps
$185
21 days
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The kit is designed to isolate total (genomic, mitochondrial, and viral) DNA from biological samples using spin columns. The kit includes all the necessary components. First, cell lysis is performed using Lysis Solution BB, and Proteinase K. Lysis Solution BB allows selective DNA binding to the membrane in the column. In the following step, a series of washings of bound DNA is performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the genomic DNA is eluted with Elution Buffer or, if necessary, with deionized water.

Kit Specifications:

  • Samples: whole blood, buccal swabs, leukocytes, cultured mammalian cells
  • Operation time: 30 minutes
  • Column binding capacity: 20 µg DNA
  • DNA typical yield: whole blood (100 µL) – 1–3 µg, buccal swab – 0.5–2 µg, cultured cells (1 × 106) – 3–6 µg
  • DNA quality: 30–50 kb in size, OD 260/280 1.7–1.8

The isolated DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.

LumiSpin BB, DNA Isolation Spin Kit for Blood and Buccal Swab, 21553

Ready-to-use kit manual

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Ready-to-use kit for efficient purification of genomic DNA from plant and animal tissues and organs, whole blood, buccal swabs, leukocytes, cultured mammalian cells, and gram-negative bacteria.

General properties

Storage conditions: Store and transport at room temperature.
MSDS: Download

Kit

Storage time in months: 12

References

  1. Ikonnikova, A.; Morozova, A.; Antonova, O.; Ochneva, A.; Fedoseeva, E.; Abramova, O.; Emelyanova, M.; Filippova, M.; Morozova, I.; Zorkina, Y.; Syunyakov, T.; Andryushchenko, A.; Andreuyk, D.; Kostyuk, G.; Gryadunov, D. Evaluation of the Polygenic Risk Score for Alzheimer’s Disease in Russian Patients with Dementia Using a Low-Density Hydrogel Oligonucleotide Microarray. International Journal of Molecular Sciences, 2023, 24(19), 14765. doi: 10.3390/ijms241914765
  2. Ikonnikova, A.; Rodina, T.; Dmitriev, A.; Melnikov, E.; Kazakov, R.; Nasedkina, T. The Influence of the CES1 Genotype on the Pharmacokinetics of Enalapril in Patients with Arterial Hypertension. Journal of Personalized Medicine, 2022, 12(4), 580. doi: 10.3390/jpm12040580
  3. Ikonnikova, A.; Kazakov, R.; Rodina, T.; Dmitriev, A.; Melnikov, E.; Zasedatelev, A.; Nasedkina, T. The Influence of Structural Variants of the CES1 Gene on the Pharmacokinetics of Enalapril, Presumably Due to Linkage Disequilibrium with the Intronic Rs2244613. Genes, 2022, 13(12), 2225. doi: 10.3390/genes13122225
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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