AF568 is a synthetic fluorophore. The excitation peak of AF568 lies at 572 nm and its emission peak is at 598 nm. It can be excited using a 561 nm laser. AF568 is spectrally similar to TF4 (Tide Fluor™ 4), and sulfo-Cyanine3.5 dyes.
As AF568 NHS Ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.
BDP 581/591 is a multi purpose fluorophore, that can also be used to probe reactive oxygen species (ROS). This is a carboxylic acid derivative with a free COOH group.
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General properties
Appearance:
dark colored solid
Mass spec M+ increment:
676.1
Molecular weight:
994.18
Molecular formula:
C49H63N5O13S2
Solubility:
good in water, DMSO, DMF
Quality control:
NMR 1H, HPLC-MS (80%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.