AF 568 NHS ester

Cat. # Quantity Price Lead time
14820 1 mg $125 in stock
24820 5 mg $325 in stock
44820 25 mg $720 in stock
54820 50 mg $1325 in stock
64820 100 mg $2090 in stock
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AF 568 is a synthetic fluorophore. The excitation peak of AF 568 lies at 572 nm and its emission peak is at 598 nm. It can be excited using a 561 nm laser. AF 568 is spectrally similar to  TF4 (Tide Fluor™ 4), and sulfo-Cyanine3.5 dyes.

As AF 568 NHS ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.

Absorption and emission spectra of AF 568

Absorption and emission spectra of AF 568

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sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

AF 568 azide

Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.

General properties

Appearance: dark colored solid
Mass spec M+ increment: 676.1
Molecular weight: 994.18
Molecular formula: C49H63N5O13S2
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 572
ε, L⋅mol−1⋅cm−1: 94238
Emission maximum, nm: 598
Fluorescence quantum yield: 0.912
CF260: 0.4
CF280: 0.32

Product citations

  1. Cepeda Diaz, A.K.; Rudlaff, R.M.; Farringer, M.; Dvorin, J.D. Essential function of alveolin Pf IMC1g in the Plasmodium falciparum asexual blood stage. mBio, 2023, 14(5), e01507-23. doi: 10.1128/mbio.01507-23
  2. Khramtsov, Y.V.; Ulasov, A.V.; Slastnikova, T.A.; Rosenkranz, A.A.; Lupanova, T.N.; Georgiev, G.P.; Sobolev, A.S. Modular Nanotransporters Delivering Biologically Active Molecules to the Surface of Mitochondria. Pharmaceutics, 2023, 15(12), 2687. doi: 10.3390/pharmaceutics15122687
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