AF 532 NHS ester

Cat. # Quantity Price Lead time
1C820 1 mg $140 in stock
2C820 5 mg $450 in stock
4C820 25 mg $1460 in stock
5C820 50 mg $1890 in stock
6C820 100 mg $2640 in stock
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Activated NHS-ester of AF 532 dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

AF 532 is a bright, photostable, and hydrophilic fluorophore emitting in the yellow-green channel, an alternative to HEX, JOE, and SIMA. The dye is useful in super-resolution microscopy, particularly in stochastic optical reconstruction microscopy (STORM), as an activator in nSTORM and a reporter in dSTORM.

Absorption and emission spectra of AF 532

Absorption and emission spectra of AF 532

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General properties

Appearance: dark red powder
Molecular weight: 824.96
Molecular formula: C40H48N4O11S2
Solubility: water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 524
ε, L⋅mol−1⋅cm−1: 80500
Emission maximum, nm: 547
Fluorescence quantum yield: 0.6
CF260: 0.17
CF280: 0.11
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