AF 488 NHS ester

Cat. # Quantity Price Lead time
11820 1 mg $125 in stock
21820 5 mg $325 in stock
31820 10 mg $480 in stock
41820 25 mg $950 in stock
51820 50 mg $1625 in stock
61820 100 mg $2090 in stock
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AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, otherwise, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF 488.

This NHS ester is an amine-reactive dye; it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: dark orange solid
Molecular weight: 732.74
Molecular formula: C31H32N4O13S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10

Product citations

  1. Schöpf, F.; Marongiu, G.L.; Milaj, K.; Sprink, T.; Kikhney, J.; Moter, A.; Roderer, D. Structural basis of Fusobacterium nucleatumadhesin Fap2 interaction with receptors on cancer and immune cells. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.28.582045
  2. Białobrzewski, M.K.; Klepka, B.P.; Michaś, A.; Cieplak-Rotowska, M.K.; Staszałek, Z.; Niedźwiecka, A. Diversity of hydrodynamic radii of intrinsically disordered proteins. European Biophysics Journal, 2023, 52, 607-618. doi: 10.1007/s00249-023-01683-8
  3. Makri Pistikou, A.-M.; Cremers, G.A.O.; Nathalia, B.L.; Meuleman, T.J.; Bögels, B.W.A.; Eijkens, B.V.; de Dreu, A.; Bezembinder, M.T.H.; Stassen, O.M.J.A.; Bouten, C.C.V.; Merkx, M.; Jerala, R.; de Greef, T.F.A. Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells. Nature Communications, 2023, 14, 7001. doi: 10.1038/s41467-023-42810-5
  4. Wiest, M.J.; Baert, L.; Gu, C.; Gayler, K.M.; Ham, H.; Gorvel, L.; Keddis, M.T.; Griffing, L.W.; Joo, H.M.; Gorvel, J.-P.; Billadeau, D.D.; Kane, R.R.; Oh, S.K. Endosomal trafficking inhibitor EGA can control TLR7-mediated IFNα expression by human plasmacytoid dendritic cells. Frontiers in Immunology, 2023, 14, 1202197. doi: 10.3389/fimmu.2023.1202197
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