AF488 NHS ester

Cat. # Quantity Price Lead time
11820 1 mg $110.00 in stock
21820 5 mg $290.00 in stock
41820 25 mg $690.00 in stock
51820 50 mg $1270.00 in stock
61820 100 mg $1990.00 in stock

AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.

This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Absorption and emission spectra of AF488

Absorption and emission spectra of AF488

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General properties

Appearance: dark orange solid
Molecular weight: 732.74
Molecular formula: C31H32N4O13S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10

Product citations

  1. Eliseev, I.E.; Ukrainskaya, V.M.; Yudenko, A.N.; Mikushina, A.D.; Shmakov, S.V.; Afremova, A.I.; Ekimova, V.M.; Vronskaia, A.A.; Knyazev, N.A.; Shamova, O.V. Targeting ErbB3 Receptor in Cancer with Inhibitory Antibodies from Llama. Biomedicines, 2021, 9(9), 1106. doi: 10.3390/biomedicines9091106
  2. Huang, D.; Yue, F.; Qiu, J.; Deng, M.; Kuang, S. Polymeric nanoparticles functionalized with muscle-homing peptides for targeted delivery of phosphatase and tensin homolog inhibitor to skeletal muscle. Acta Biomaterialia, 2020, 118, 196–206. doi: 10.1016/j.actbio.2020.10.009
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