AF488 NHS ester

Cat. # Quantity Price Lead time
11820 1 mg $110.00 5 days
21820 5 mg $290.00 5 days
41820 25 mg $690.00 21 days
51820 50 mg $1270.00 21 days
61820 100 mg $1990.00 21 days

AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.

This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Absorption and emission spectra of AF488

Absorption and emission spectra of AF488

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General properties

Appearance: dark orange solid
Molecular weight: 732.74
Molecular formula: C31H32N4O13S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid)
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
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