AF488 NHS ester

Cat. # Quantity Price Lead time
11820 1 mg $110.00 in stock
21820 5 mg $290.00 in stock
41820 25 mg $690.00 5 days
51820 50 mg $1270.00 in stock
61820 100 mg $1990.00 10 days

AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.

This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Absorption and emission spectra of AF488

Absorption and emission spectra of AF488

Customers also purchased with this product

DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.

Sulfo-Cyanine5 azide

Water soluble sulfo-Cyanine5 fluorescent dye azide for Click Chemistry.

TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.

General properties

Appearance: dark orange solid
Molecular weight: 732.74
Molecular formula: C31H32N4O13S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91

Product citations

  1. Huang, D.; Yue, F.; Qiu, J.; Deng, M.; Kuang, S. Polymeric nanoparticles functionalized with muscle-homing peptides for targeted delivery of phosphatase and tensin homolog inhibitor to skeletal muscle. Acta Biomaterialia, in press. doi: 10.1016/j.actbio.2020.10.009
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.