AF 488 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11880 | 1 mg | $140 | in stock | |
| 21880 | 5 mg |
$450
|
in stock | |
| 41880 | 25 mg |
$1460
|
in stock | |
| 51880 | 50 mg | please inquire | in stock | |
| 61880 | 100 mg | please inquire | in stock |
AF 488 C6 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 488 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.
AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for sensitive proteins and antibodies labeling. The dye is useful for numerous demanding applications, including microscopy.
AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products otherwise. We offer an isomerically pure 5-AF 488.
Absorption and emission spectra of AF 488
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General properties
| Appearance: | red-orange powder |
| Molecular weight: | 734.69 |
| Molecular formula: | C31H31N4NaO12S2 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
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