AF 488 maleimide

Cat. # Quantity Price Lead time
11880 1 mg $140 in stock
21880 5 mg $450 in stock
41880 25 mg $1460 in stock
51880 50 mg please inquire in stock
61880 100 mg please inquire in stock
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AF 488 C6 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 488 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.

AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for sensitive proteins and antibodies labeling. The dye is useful for numerous demanding applications, including microscopy.

AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products otherwise. We offer an isomerically pure 5-AF 488.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

General properties

Appearance: red-orange powder
Molecular weight: 734.69
Molecular formula: C31H31N4NaO12S2
Solubility: good in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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