AF 488 amine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118C0 | 1 mg | $125 | in stock | |
| 218C0 | 5 mg | $325 | in stock | |
| 318C0 | 10 mg |
$480
|
in stock | |
| 418C0 | 25 mg |
$950
|
in stock | |
| 518C0 | 50 mg |
$1625
|
in stock | |
| 618C0 | 100 mg |
$2090
|
in stock |
AF 488 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the green region (at 519 nm).
Conjugates of biomolecules with AF 488 can be used in flow cytometry, for example in multicolor staining. AF 488 is also used in cell microscopy due to its high photostability.
AF 488 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.
Absorption and emission spectra of AF 488
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Sulfonated Cyanine5 amine derivative for the conjugation with electrophiles and enzymatic transamination labeling.General properties
| Appearance: | orange crystals |
| Molecular weight: | 733.87 |
| Molecular formula: | C33H43N5O10S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
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