AF 430 hydrazide

Cat. # Quantity Price Lead time
10870 1 mg $110 in stock
20870 5 mg $210 in stock
40870 25 mg $410 in stock
50870 50 mg $695 in stock
60870 100 mg $1190 in stock
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AF 430 is a fluorescent dye with an excitation maximum at 430 nm and an emission maximum at 542 nm. AF 430 is one of the few dyes that absorb between 400 nm and 450 nm. AF 430 fluorescence is photostable and pH-insensitive in a broad range of pH values.

This product is a hydrazide derivative of AF 430 dye. Hydrazides efficiently react with aldehydes and ketones, resulting in hydrazones, so that this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with hydrazides). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can also be conjugated with hydrazides in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Absorption and emission spectra of AF 430

Absorption and emission spectra of AF 430

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General properties

Appearance: yellow powder
Mass spec M+ increment: 499.1
Molecular weight: 517.52
Molecular formula: C22H26N3F3O6S
Solubility: good in DMF, DMSO, water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
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