AF430 is a fluorescent dye structurally and functionally analogous to Alexa Fluor™ 430, which belongs to the popular Alexa Fluor™ family of fluorescent dyes. Its excitation maximum is at 430 nm, emission maximum is at 542 nm, and Stokes shift is 112 nm. Like Alexa Fluor™ 430, AF430 exhibits high photostability and pH-insensitive fluorescence. The dye can be excited by 405 nm violet laser or 445 nm laser.
AF430 carboxylic acid is a non-reactive form of AF430 dye that can be used for the analysis of labeled samples — as a reference standard in procedures where AF430 dye conjugates are involved.
AF430 is a dye emitting in green-yellow range. A dye is useful for flow cytometry.
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General properties
Appearance:
yellow solid
Molecular weight:
541.58
Molecular formula:
C22H23NF3KO7S
Solubility:
good in DMF, DMSO, water
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.