AF430 amine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 108C0 | 1 mg | $110.00 | in stock | |
| 208C0 | 5 mg | $290.00 | in stock | |
| 408C0 | 25 mg |
$690.00
|
in stock | |
| 508C0 | 50 mg |
$1270.00
|
in stock | |
| 608C0 | 100 mg |
$1990.00
|
in stock |
AF430 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the yellow-green region (at 542 nm).
Conjugates of biomolecules with AF430 can be used in flow cytometry, for example in multicolor staining. AF430 is also used in cell microscopy due to its high photostability.
AF430 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.
Absorption and emission spectra of AF430
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AF430 NHS ester
AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.General properties
| Appearance: | yellow solid |
| Molecular weight: | 601.68 |
| Molecular formula: | C28H38N3F3O6S |
| IUPAC name: | (9-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-8,8-dimethyl-2-oxo-4-(trifluoromethyl)-8,9-dihydro-2H-pyrano[3,2-g]quinolin-6-yl)methanesulfonate |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
| CF260: | 0.06 |
| CF280: | 0.06 |
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