AF430 amine

Cat. # Quantity Price Lead time
108C0 1 mg $110.00 in stock
208C0 5 mg $290.00 in stock
408C0 25 mg $690.00 in stock
508C0 50 mg $1270.00 in stock
608C0 100 mg $1990.00 in stock

AF430 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the yellow-green region (at 542 nm).

Conjugates of biomolecules with AF430 can be used in flow cytometry, for example in multicolor staining. AF430 is also used in cell microscopy due to its high photostability.

AF430 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.

Absorption and emission spectra of AF430

Absorption and emission spectra of AF430

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AF430 NHS ester

AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.

General properties

Appearance: yellow solid
Molecular weight: 601.68
Molecular formula: C28H38N3F3O6S
IUPAC name: (9-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-8,8-dimethyl-2-oxo-4-(trifluoromethyl)-8,9-dihydro-2H-pyrano[3,2-g]quinolin-6-yl)methanesulfonate
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06
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