Pyrene phosphoramidite dU
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11460 | 100 mg | $240 | in stock | |
| 21460 | 250 mg |
$460
|
in stock | |
| 41460 | 1 g |
$890
|
in stock | |
| 61460 | 5 g | please inquire | in stock |
Pyrene is a polycyclic aromatic hydrocarbon that is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on the fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about the site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows attaching pyrene fragment to 5', internal, or 3' position (using universal support).
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
Absorption and emission spectra of pyrene fluorophore
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sulfo-Cyanine5 maleimide
Thiol reactive maleimide, water soluble cyanine dye for Cy5 channel.Pyrene azide 1
Reagent for fluorescent labeling with pyrene polycyclic aromatic hydrocarbon via click chemistry.General properties
| Appearance: | yellowish foam |
| Molecular weight: | 955.04 |
| CAS number: | 199920-17-7 |
| Molecular formula: | C57H55N4O8P |
| Solubility: | good in dichloromethane and acetonitrile |
| Quality control: | NMR 1H (95%), 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 260; 282; 365; 392 |
| ε, L⋅mol−1⋅cm−1: | 12600; 21900; 16000; 14200 |
| Emission maximum, nm: | 460 |
| Fluorescence quantum yield: | ~0.1 |
Oligo synthesis details
| Diluent: | acetonitrile |
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