Cyanine5 maleimide

Cat. # Quantity Price Lead time Buy this product
13080 1 mg 110.00$ in stock
23080 5 mg 210.00$ in stock
43080 25 mg 410.00$ in stock
53080 50 mg 695.00$ in stock
63080 100 mg 1190.00$ in stock

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

We recommend to use water soluble sulfo-Cyanine5 maleimide for the labeling of antibodies and sensitive proteins.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

Cy5 excitation and emission spectra

General properties

Appearance: dark blue powder
Molecular weight: 641.24
Molecular formula: C38H45ClN4O3
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

Product citations

  1. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology, 2015, 1266, 185–198. doi: 10.1007/978-1-4939-2272-7_13
  2. Leonard, J.D.; Narlikar, G.J. A Nucleotide-Driven Switch Regulates Flanking DNA Length Sensing by a Dimeric Chromatin Remodeler. Molecular Cell, 2015, 57(5), 850–859. doi: 10.1016/j.molcel.2015.01.008
  3. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  4. Damayanti, N.P.; Parker, L.L.; Irudayaraj, J.M.K. Fluorescence Lifetime Imaging of Biosensor Peptide Phosphorylation in Single Live Cells. Angewandte Chemie International Edition, 2013, 52(14), 3931-3934. doi: 10.1002/anie.201209303
  5. Hartley, M.D.; Schneggenburger, P.E.; Imperiali, B. Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities. Proceedings of the National Academy of Sciences, 2013, 110(52), 20863-20870. doi: 10.1073/pnas.1320852110
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