Cyanine5 maleimide

Cat. # Quantity Price Lead time
13080 1 mg $110.00 in stock
23080 5 mg $210.00 in stock
43080 25 mg $410.00 in stock
53080 50 mg $695.00 in stock
63080 100 mg $1190.00 in stock

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

We recommend to use water soluble sulfo-Cyanine5 maleimide for the labeling of antibodies and sensitive proteins.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

Cy5 excitation and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 641.24
Molecular formula: C38H45ClN4O3
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

Product citations

  1. Noriega, R.; Finley, D.T.; Haberstroh, J.; Geissler, P.L.; Francis, M.B.; Ginsberg, N.S. Manipulating Excited-State Dynamics of Individual Light-Harvesting Chromophores through Restricted Motions in a Hydrated Nanoscale Protein Cavity. The Journal of Physical Chemistry B, 2015, 119(23), 6963–6973. doi: 10.1021/acs.jpcb.5b03784
  2. Leonard, J.D.; Narlikar, G.J. A Nucleotide-Driven Switch Regulates Flanking DNA Length Sensing by a Dimeric Chromatin Remodeler. Molecular Cell, 2015, 57(5), 850–859. doi: 10.1016/j.molcel.2015.01.008
  3. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology, 2015, 1266, 185–198. doi: 10.1007/978-1-4939-2272-7_13
  4. Lipchik, A.M.; Perez, M.; Cui, W.; Parker, L.L. Multicolored, Tb3+-Based Antibody-Free Detection of Multiple Tyrosine Kinase Activities. Analytical Chemistry, 2015, 87(15), 7555–7558. doi: 10.1021/acs.analchem.5b02233
  5. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  6. Damayanti, N.P.; Parker, L.L.; Irudayaraj, J.M.K. Fluorescence Lifetime Imaging of Biosensor Peptide Phosphorylation in Single Live Cells. Angewandte Chemie International Edition, 2013, 52(14), 3931-3934. doi: 10.1002/anie.201209303
  7. Hartley, M.D.; Schneggenburger, P.E.; Imperiali, B. Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities. Proceedings of the National Academy of Sciences of the U.S.A., 2013, 110(52), 20863-20870. doi: 10.1073/pnas.1320852110
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