Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13080 | 1 mg |
$179.90
|
in stock | |
| 23080 | 5 mg |
$299.90
|
in stock | |
| 43080 | 25 mg |
$569.90
|
in stock | |
| 53080 | 50 mg |
$829.90
|
in stock | |
| 63080 | 100 mg |
$1099.00
|
in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cyanine5 excitation and emission spectra
Recommended protocol
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 605.8 |
| CAS number: | 1437872-46-2 (without anion) |
| Molecular formula: | C38H45N4O3 |
| IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Uckelmann, M.; Levina, V.; Taveneau, C.; Ng, X. H.; Pandey, V.; Martinez, J.; Mendiratta, S.; Houx, J.; Boudes, M.; Venugopal, H.; Trépout, S.; Fulcher, A. J.; Zhang, Q.; Flanigan, S.; Li, M.; Sierecki, E.; Gambin, Y.; Das, P. P.; Bell, O.; de Marco, A.; Davidovich, C. Dynamic PRC1–CBX8 Stabilizes a Porous Structure of Chromatin Condensates. Nat Struct Mol Biol, 2025, 32(3), 520–530. doi: 10.1038/s41594-024-01457-6
- Matthews, M. D.; Saffari, A.; Mukul, N.; Hai, L.; Naguib, N.; Wiesner, U. B.; Lewis, K. J. Real-Time Visualization and Modulation of Endocytic Dynamics in Osteocytes in Vivo. Sci Rep, 2025, 15(1), 31454. doi: 10.1038/s41598-025-05735-1
- He, Y.; Zalenski, N.; Stephenson, A. A.; Raper, A. T.; Ghimire, C.; Suo, Z. Conformational Transitions of Streptococcus Pyogenes Cas9 Induced by Salt and Single-Guide RNA Binding. Journal of Biological Chemistry, 2025, 301(2), 108120. doi: 10.1016/j.jbc.2024.108120
- Huang, L.; Liu, P.; Du, Y.; Pan, D.; Lee, A.; Wolfe, S.A.; Wang, Y.-X. A brown fat-enriched adipokine, ASRA, is a leptin receptor antagonist that stimulates appetite. bioRxiv, 2024, preprint. doi: 10.1101/2023.09.12.557454
Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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