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Alkyne Phosphoramidite, 5'-terminal
| Lot No | Quantity | Price | |
|---|---|---|---|
| 22260 | 250 mg | $ 230.00 | |
| 42260 | 1 g | $ 450.00 | |
| 62260 | 5 g | $ 1990.00 |
Phosphoramidite for the synthesis of alkyne-modified oligonucleotides containing 5'-terminal modification. Oligonucleotides can be used for Click Chemistry modification (see or download protocol).
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites:
- Solid compound - easy to dispense
- Secondary amidite derivative, more stable in solution
- Ideal for oligo houses doing Click Chemistry post-modification, and offering alkyne oligos
| General properties | |
|---|---|
| Appearance: | white crystalline solid |
| Molar weight: | 409.50 |
| Oligonucleotide synthesis conditions | |
| Synthesizer compatibility | all synthesizers using phosphoramidite chemistry |
| Coupling time | 5 min recommended |
| Deblock conditions | Standard (6h in aq. ammonia at 60°C) |
| Oligo purification | PAAG electrophoresis or HPLC |
| Oligo storage | not different from standart oligonucleotides |
| Quality control | |
| NMR (1H, 31P): | 95+% |
| Functional testing (oligo synthesis + oligo modification with CuAAC): | pass |
| Recommended protocols | |
| Click-chemistry labeling of oligonucleotides and DNA | HTML | PDF |
| Storage conditions | |
| Store at -20°C. Desiccate. | |
Order now. Receive within 3-8 days in any place around the world.
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