sulfo-Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13320 1 mg $91 in stock
23320 5 mg $119 in stock
43320 25 mg $345 in stock
53320 50 mg $557 in stock
63320 100 mg $890 in stock
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Water-soluble Cyanine5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is, therefore, particularly useful for the labeling of proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.

sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores, which is compatible with various equipment such as plate readers, microscopes, and imagers.

This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.

Can be used as a replacement for Cy5®, DyLight 649 for all applications.

sulfo-Cyanine5 absorbance and emission spectra

sulfo-Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 777.95
CAS number: 2230212-27-6; 146368-14-1 (N-Ethyl)
Molecular formula: C36H40N3KO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Geng, T.; Paek, S.Y.; Leung, E.; Chamley, L.W.; Wu, Z. Comparing extracellular vesicles from four different cell origins for intracellular drug delivery to pancreatic cancer cells: Small or large vesicles?. Journal of drug delivery science and technology, 2024, 93, 105416. doi: 10.1016/j.jddst.2024.105416
  2. Medina, S.; Miller, M. Synthetic Colonic Mucus Enables the Development of Modular Microbiome Organoids. Research Square, 2023. doi: 10.21203/rs.3.rs-3164407/v1
  3. Liu, Z.; Guo, K.; Yan, L.; Zhang, K.; Wang, Y.; Ding, X.; Zhao, N.; Xu, F.-J. Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms. Nature Communications, 2023, 14, 5132. doi: 10.1038/s41467-023-40830-9
  4. Fischbach, A.; Johns, A.; Schneider, K.L.; Hao, X.; Tessarz, P.; Nyström, T. Artificial Hsp104-mediated systems for re-localizing protein aggregates. Nature Communications, 2023, 14, 2663. doi: 10.1038/s41467-023-37706-3
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