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Sulfo-Cy5 NHS ester
| Lot No | Quantity | Price | |
|---|---|---|---|
| 13320 | 1 mg | $ 160.00 | |
| 23320 | 5 mg | $ 410.00 | |
| 43320 | 25 mg | $ 850.00 | |
| 53320 | 50 mg | $ 1500.00 | |
| 63320 | 100 mg | $ 2450.00 |
Water soluble Cy5 succinimidyl ester, for the labeling of various amine-containing molecules in aqueous phase, without use of organic co-solvent.
This dye is highly hydrophilic and water-soluble. Non-sulfonated analog is available.
Can be used as a replacement for Alexa Fluor 647, DyLight 649 for all applications.
| General properties | |
|---|---|
| Appearance: | dark blue powder |
| Solubility: | Good in water and polar organic solvents (DMF, DMSO), moderate in alcohols and acetonitrile, poor in organic solvents (DCM, chloroform) |
| Molar weight: | 761.84 |
| Mass spec M+ increase: | 624.8 |
| Spectral properties | |
| Cy5 excitation maximum, λmaxex, nm: | 646 |
| Cy5 extinction at λmaxex | 271,000 |
| Cy5 emission maximum, λmaxfluor, nm: | 662 |
| Cy5 fluorescence quantum yield: | 0.2 |
| Quality control | |
| NMR: | 95+% |
| TLC: | pure |
| Functional testing (reaction with amine): | pass |
| Recommended protocols | |
| NHS ester labeling of amino biomolecules | HTML | PDF |
| Storage conditions | |
| Store NHS esters at -20°C in the dark. Desiccate. | |
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