Cy5 NHS ester
| Cat. # | Quantity | Price | Stock
Stock items are shipped either today (for orders placed by 12AM EST), or tomorrow. Backordered items are typically shipped within 10 days. 
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Buy this product |
|---|---|---|---|---|
| 13020 | 1 mg | 110.00$ | in stock | |
| 23020 | 5 mg | 210.00$ | in stock | |
| 43020 | 25 mg | 410.00$ | in stock | |
| 53020 | 50 mg | 695.00$ | in stock | |
| 63020 | 100 mg | 1190.00$ | in stock |
During last years, Cy5 flurophore has become an incredibly popular label in life science research and diagnostics. Fluorophore emission has maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. Dye color is very intense, therefore quantity as small as 1 nanomol can be detected in gel electrophoresis by naked eye.
This Cy5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble Cy5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.
Cy5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cy5 analogs exist - Cy5 NHS ester can replace activated esters of Alexa Fluor 647, and DyLight 649.
Recommended protocol
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 616.19 |
| Molecular formula: | C36H42ClN3O4 |
| Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 646 |
| Extinction coefficient at excitation maximum, Lmol-1cm-1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
