Cyanine5 NHS ester

Cat. # Quantity Price Lead time
13020 1 mg $110.00 in stock
23020 5 mg $210.00 in stock
43020 25 mg $410.00 in stock
53020 50 mg $695.00 in stock
63020 100 mg $1190.00 in stock

During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.

Cy5 excitation and emission spectra

Customers also purchased with this product

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

Cyanine7.5 maleimide

Cyanine7.5 maleimide is a thiol reactive near infrared dye.
Add this product to your cart and
get free express delivery

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

General properties

Appearance: dark blue powder
Molecular weight: 616.19
CAS number: 1032678-42-4, 350686-88-3
Molecular formula: C36H42ClN3O4
Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

Product citations

  1. Bloch, M.B.D.; Yavin, E.; Nissan, A.; Ariel, I.; Kenett, R.; Brass, D.; Rubinstein, A. The effect of linker type and recognition peptide conjugation chemistry on tissue affinity and cytotoxicity of charged polyacrylamide. Journal of Controlled Release, in press. doi: 10.1016/j.jconrel.2016.06.038
  2. Wilkinson, N.; Metaxas, A.; Ruud, E.; Raethke, E.; Wickramaratne, S.; Reineke, T.M.; Dutcher, C.S. Internal structure visualization of polymer – clay flocculants using fluorescence. Colloids and Interface Science Communications, 2016, 10–11, 1–5. doi: 10.1016/j.colcom.2016.03.002
  3. Chen, K.; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327. doi: 10.1016/j.ijpharm.2016.05.060
  4. Wu, F.; Liu, C.; Chen, Y.; Yang, S.; Xu, J.; Huang, R.; Wang, X.; Li, M.; Liu, W.; Mao, W.; Zhou, X. Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe. Sensors and Actuators B: Chemical, 2016, 236, 268–275. doi: 10.1016/j.snb.2016.05.162
  5. Ray, J.; Shin, I.; Ilgu, M.; Bendickson, L.; Gupta, V.; Kraus, G.A.; Nilsen-Hamilton, M. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters. Methods in Enzymology, 2016, 572, 193–213. doi: 10.1016/bs.mie.2016.02.028
  6. Hartley, J.M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeček, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59. doi: 10.1016/j.jconrel.2016.02.005
  7. Xu, X.; Li, L.; Zhou, Z.; Sun, W.; Huang, Y. Dual-pH responsive micelle platform for co-delivery of axitinib and doxorubicin. International Journal of Pharmaceutics, 2016, 507(1–2), 50–60. doi: 10.1016/j.ijpharm.2016.04.060
  8. Tran, T.-H.; Krishnan, S.; Amiji, M.M. MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects. PLoS One, 2016, 11(5), e0152024. doi: 10.1371/journal.pone.0152024
  9. Liu, C.; Stonestrom, A.J.; Christian, T.; Yong, J.; Takase, R.; Hou, Y.-M.; Yang, X. Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction. Journal of Biological Chemistry, 2016, 291(19), 10426–10436. doi: 10.1074/jbc.M115.697789
  10. Li, L.; Sun, W.; Zhang, Z.; Huang, Y. Time-staggered delivery of docetaxel and H1S6A,F8A peptide for sequential dual-strike chemotherapy through tumor priming and nuclear targeting. Journal of Controlled Release, 2016, 232, 62–74. doi: 10.1016/j.jconrel.2016.04.021
  11. Leenders, J.; Baker, M.B.; Pijpers, I.; Lafleur, R.; Albertazzi, L.; Palmans, A.R.A.; Meijer, E.W. Supramolecular polymerisation in water; elucidating the role of hydrophobic and hydrogen-bond interactions. Soft Matter, 2016, 12, 2887–2893. doi: 10.1039/c5sm02843d
  12. Liu, X.; Zhang, P.; He, D:; Rödl, W.; Preiß, T.; Rädler, J.O.; Wagner, E.; Lächelt, U. pH-reversible cationic RNase A conjugates for enhanced cellular delivery and tumor cell killing. Biomacromolecules, 2016, 17(1), 173–182. doi: 10.1021/acs.biomac.5b01289
  13. Schelpe, J.; Monté, D.; Dewitte, F.; Sixma, T.K.; Rucktooa, P. Structure of UBE2Z Enzyme Provides Functional Insight into Specificity in the FAT10 Protein Conjugation Machinery. Journal of Biological Chemistry, 2016, 291(2), 630–639. doi: 10.1074/jbc.M115.671545
  14. He, H.; Altomare, D.; Ozer, U.; Xu, H.; Creek, K.; Chen, H.; Xu, P. Cancer cell-selective killing polymer/copper combination. Biomaterials Science, 2016, 4(1), 115–120. doi: 10.1039/c5bm00325c
  15. van der Velde, J.H.M.; Oelerich, J.; Huang, J.; Smit, J.H.; Aminian Jazi, A.; Galiani, S.; Kolmakov, K.; Guoridis, G.; Eggeling, C.; Herrmann, A.; Roelfes, G.; Cordes, T. A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization. Nature Communications, 2016, 7, 10144. doi: 10.1038/ncomms10144
  16. Poolman, J.; Maity, C.; Boekhoven, J.; van der Mee, L.; le Sage, V.; Groenewold, M.; van Kasteren, S.; Versluis, F.; van Esch, J.; Eelkema, R. A toolbox for controlling the properties and functionalisation of hydrazone-based supramolecular hydrogels. Journal of Materials Chemistry B, 2016, 4, 852–858. doi: 10.1039/C5TB01870F
  17. Stephen, Z.; Dayringer, C.; Lim, J.; Revia, R.; Halbert, M.; Jeon, M.; Bakthavatsalam, A.; Ellenbogen, R.G.; Zhang, M. An Approach to Rapid Synthesis and Functionalization of Iron Oxide Nanoparticles for High Gene Transfection. ACS Applied Materials & Interfaces, 2016, 8(10), 6320–6328. doi: 10.1021/acsami.5b10883
  18. Evans, L.E.; Cheeseman, M.D.; Yahya, N.; Jones, K. Investigating Apoptozole as a Chemical Probe for HSP70 Inhibition. PLoS One, 2015, 10(10), e0140006. doi: 10.1371/journal.pone.0140006
  19. Chu, D.; Gao, J.; Wang, Z. Neutrophil-Mediated Delivery of Therapeutic Nanoparticles across Blood Vessel Barrier for Treatment of Inflammation and Infection. ACS Nano, 2015, 9(12), 11800–11811. doi: 10.1021/acsnano.5b05583
  20. Albertazzi, L.; van der Veeken, N.; Baker, M.B.; Palmans, A.R.A.; Meijer, E.W. Supramolecular copolymers with stimuli-responsive sequence control. Chemical Communications, 2015, 51(90), 16166–16168. doi: 10.1039/c5cc06951c
  21. Yang, J.; Zhang, R.; Radford, D.C.; Kopeček, J. FRET-trackable biodegradable HPMA copolymer-epirubicin conjugates for ovarian carcinoma therapy. Journal of Controlled Release, 2015, 218, 36–44. doi: 10.1016/j.jconrel.2015.09.045
  22. Wang, C.; Wang, Y.; Li, Y.; Bodemann, B.; Zhao, T.; Ma, X.; Huang, G.; Hu, Z.; DeBerardinis, R.J.; White, M.A.; Gao, J. A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nature Communications, 2015, 6, 8524. doi: 10.1038/ncomms9524
  23. Vanparijs, N.; De Coen, R.; Laplace, D.R.; Louage, B.; Maji, S.; Lybaert, L.; Hoogenboom, R.; De Geest, B. Transiently responsive protein-polymer conjugates via a grafting-from RAFT approach: for intracellular co-delivery of proteins and immune-modulators. Chemical Communications, 2015, 51, 13972–13975. doi: 10.1039/c5cc04809e
  24. Klykov, O.; Weller, M.G. Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC). Analytical Methods, 2015, 7, 6443–6448. doi: 10.1039/c5ay00042d
  25. Shi, Q.; Zhang, L.; Liu, M.; Zhang, X.; Zhang, X.; Xu, X.; Chen, S.; Li, X.; Zhang, J. Reversion of multidrug resistance by a pH-responsive cyclodextrin-derived nanomedicine in drug resistant cancer cells. Biomaterials, 2015, 67, 169–182. doi: 10.1016/j.biomaterials.2015.07.023
  26. Chen, L.; An, H.; Doyle, P.S. Synthesis of non-spherical microcapsules through controlled polyelectrolyte coating of hydrogel templates. Langmuir, 2015, 31(33), 9228–9235. doi: 10.1021/acs.langmuir.5b02200
  27. Li, L.; Sun, W.; Zhong, J.; Yang, Qi.; Zhu, X.; Zhou, Z.; Zhang Z.; Huang, Y. Multistage Nanovehicle Delivery System Based on Stepwise Size Reduction and Charge Reversal for Programmed Nuclear Targeting of Systemically Administered Anticancer Drugs. Advanced Functional Materials, 2015, 25(26), 4101–4113. doi: 10.1002/adfm.201501248
  28. Baker, Matthew B. and Albertazzi, Lorenzo and Voets, Ilja K. and Leenders, Christianus M.A. and Palmans, Anja R.A. and Pavan, Giovanni M. and Meijer, E.W. Consequences of chirality on the dynamics of a water-soluble supramolecular polymer. Nature Communications, 2015, 6, 6234. doi: 10.1038/ncomms7234
  29. Unciti-Broceta, J.D.; Cano-Cortés, V.; Altea-Manzano, P.; Pernagallo, S.; Díaz-Mochón, J.J.; Sánchez-Martín, R.M. Number of Nanoparticles per Cell through a Spectrophotometric Method – A key parameter to Assess Nanoparticle-based Cellular Assays. Scientific Reports, 2015, 5, 10091. doi: 10.1038/srep10091
  30. Medina, S.H.; Schneider, J.P. Cancer cell surface induced peptide folding allows intracellular translocation of drug. Journal of Controlled Release, 2015, 209, 317–326. doi: 10.1016/j.jconrel.2015.05.267
  31. Pereira, P.M.; Almada, P.; Henriques, R. High-content 3D multicolor super-resolution localization microscopy. Methods in Cell Biology, 2015, 125, 95–117. doi: 10.1016/bs.mcb.2014.10.004
  32. Chiang, W.-L.; Lin, T.-T.; Sureshbabu, R.; Chia, W.-T.; Hsiao, H.-C.; Liu, H.-Y.; Yang, C.-M.; Sung, H.-W. A rapid drug release system with a NIR light-activated molecular switch for dual-modality photothermal/antibiotic treatments of subcutaneous abscesses. Journal of Controlled Release, 2015, 199, 53–62. doi: 10.1016/j.jconrel.2014.12.011
  33. Geertsema, H.J.; Duderstadt, K.E.; van Oijen, A.M. Single-molecule observation of prokaryotic DNA replication. Methods in Molecular Biology, 2015, 1300, 219–238. doi: 10.1007/978-1-4939-2596-4_14
  34. Nooney, R.; O’Connell, C.; Roy, S.; Boland, K.; Keegan, G.; Kelleher, S.; Daniels, S.; McDonagh, C. Synthesis and characterisation of far-red fluorescent cyanine dye doped silica nanoparticles using a modified microemulsion method for application in bioassays. Sensors and Actuators B: Chemical, 2015, 221, 470–479. doi: 10.1016/j.snb.2015.06.117
  35. Zhang, Z.; Kenny, S.J.; Hauser, M.; Li, W.; Xu, K. Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy. Nature Methods, 2015, 12(10), 935–938. doi: 10.1038/nmeth.3528
  36. Chen, K.; Xu, X.; Guo, J.W.; Zhang, X.; Han, S.; Wang, R.; Li, X.; Zhang, J. Enhanced Intracellular Delivery and Tissue Retention of Nanoparticles by Mussel-Inspired Surface Chemistry. Biomacromolecules, 2015, 16(11), 3574–3583. doi: 10.1021/acs.biomac.5b01056
  37. Dogandzhiyski, P.; Ghidini, A.; Danneberg, F.; Strömberg, R.; Göbel, M.W. Studies on Tris(2-aminobenzimidazole)-PNA Based Artificial Nucleases: A Comparison of Two Analytical Techniques. Bioconjugate Chemistry, 2015, 26(12), 2514–2519. doi: 10.1021/acs.bioconjchem.5b00534
  38. Bird, G.H., Boyapalle, S.; Wong, T.; Opoku-Nsiah, K.; Bedi, R.; Crannell, W.C.; Perry, A.F.; Nguyen, H.; Sampayo, V.; Devareddy, A.; Mohapatra, S.; Mohapatra S.S.; Walensky, L.D. Mucosal delivery of a double-stapled {RS}V peptide prevents nasopulmonary infection. Journal of Clinical Investigation, 2014, 124(5), 2113–2124. doi: 10.1172/jci71856
  39. Basu, R.; Lai, L.-T.; Meng, Z.; Wu, J.; Shao, F.; Zhang, L.-F. Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH. PLoS ONE, 2014, 9(9), e107425. doi: 10.1371/journal.pone.0107425
  40. Bříza, T.; Rimpelová, S.; Králová, J.; Záruba, K.; Kejík, Z.; Ruml, T.; Martásek, P.; Král, V. Pentamethinium fluorescent probes: The impact of molecular structure on photophysical properties and subcellular localization. Dyes and Pigments, 2014, 107, 51-59. doi: 10.1016/j.dyepig.2013.12.021
  41. Bamrungsap, S.; Apiwat, C.; Chantima, W.; Dharakul, T.; Wiriyachaiporn, N. Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles. Microchimica Acta, 2014, 181(1–2), 223-230. doi: 10.1007/s00604-013-1106-4
  42. Duellberg, C.; Trokter, M.; Jha, R.; Sen, I.; Steinmetz, M.O.; Surrey, T. Reconstitution of a hierarchical +TIP interaction network controlling microtubule end tracking of dynein. Nature Cell Biology, 2014, 16(8), 804-811. doi: 10.1038/ncb2999
  43. Geertsema, H.J.; Kulczyk, A.W.; Richardson, C.C.; van Oijen, A.M. Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery. Proceedings of the National Academy of Sciences of the U.S.A., 2014, 111(11), 4073-4078. doi: 10.1073/pnas.1402010111
  44. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  45. Hu, X.; Wang, Q.; Liu, Y.; Liu, H.; Qin, C.; Cheng, K.; Robinson, W.; Gray, B.D.; Pak, K.Y.; Yu, A. et al. Optical imaging of articular cartilage degeneration using near-infrared dipicolylamine probes. Biomaterials, 2014, 35(26), 7511-7521. doi: 10.1016/j.biomaterials.2014.05.042
  46. Novo, L.; Rizzo, L.Y.; Golombek, S.K.; Dakwar, G.R.; Lou, B.; Remaut, K.; Mastrobattista, E.; van Nostrum, C.F.; Jahnen-Dechent, W.; Kiessling, F. et al. Decationized polyplexes as stable and safe carrier systems for improved biodistribution in systemic gene therapy. Journal of Controlled Release, 2014, 195, 162-175. doi: 10.1016/j.jconrel.2014.08.028
  47. Zhang, Y.; Ge, C.; Zhu, C.; Salaita, K. DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nature Communications, 2014, 5, 5167-5167. doi: 10.1038/ncomms6167
  48. Chen, H.; Xiao, L.; Anraku, Y.; Mi, P.; Liu, X.; Cabral, H.; Inoue, A.; Nomoto, T.; Kishimura, A.; Nishiyama, N. et al. Polyion Complex Vesicles for Photoinduced Intracellular Delivery of Amphiphilic Photosensitizer. Journal of the American Chemical Society, 2014, 136(1), 157-163. doi: 10.1021/ja406992w
  49. Lee, S.; Ashizawa, A.T.; Kim, K.S.; Falk, D.J.; Notterpek, L. Liposomes to Target Peripheral Neurons and Schwann Cells. PLoS ONE, 2013, 8(11), e78724. doi: 10.1371/journal.pone.0078724
  50. Albertazzi, L.; Martinez-Veracoechea, F.J.; Leenders, C.M.A.; Voets, I.K.; Frenkel, D.; Meijer, E.W. Spatiotemporal control and superselectivity in supramolecular polymers using multivalency. Proceedings of the National Academy of Sciences of the U.S.A., 2013, 110(30), 12203-12208. doi: 10.1073/pnas.1303109110
  51. Cheng, M.-C.; Leske, A.T.; Matsuoka, T.; Kim, B.C.; Lee, J.; Burns, M.A.; Takayama, S.; Biteen, J.S. Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy. The Journal of Physical Chemistry B, 2013, 117(16), 4406-4411. doi: 10.1021/jp307635v
  52. Haller, A.; Altman, R.B.; Souliere, M.F.; Blanchard, S.C.; Micura, R. Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution. Proceedings of the National Academy of Sciences of the U.S.A., 2013, 110(11), 4188-4193. doi: 10.1073/pnas.1218062110
  53. He, H.; Chen, S.; Zhou, J.; Dou, Y.; Song, L.; Che, L.; Zhou, X.; Chen, X.; Jia, Y.; Zhang, J. et al. Cyclodextrin-derived pH-responsive nanoparticles for delivery of paclitaxel. Biomaterials, 2013, 34(21), 5344-5358. doi: 10.1016/j.biomaterials.2013.03.068
  54. Rimpelová, S.; Bříza, T.; Králová, J.; Záruba, K.; Kejík, Z.; Císařová, I.; Martásek, P.; Ruml, T.; Král, V. Rational Design of Chemical Ligands for Selective Mitochondrial Targeting. Bioconjugate Chemistry, 2013, 24(9), 1445-1454. doi: 10.1021/bc400291f
  55. Soulière, M.F.; Altman, R.B.; Schwarz, V.; Haller, A.; Blanchard, S.C.; Micura, R. Tuning a riboswitch response through structural extension of a pseudoknot. Proceedings of the National Academy of Sciences of the U.S.A., 2013, 110(35), E3256-E3264. doi: 10.1073/pnas.1304585110
  56. Yang, H.; Mao, H.; Wan, Z.; Zhu, A.; Guo, M.; Li, Y.; Li, X.; Wan, J.; Yang, X.; Shuai, X. et al. Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy. Biomaterials, 2013, 34(36), 9124-9133. doi: 10.1016/j.biomaterials.2013.08.022
  57. Pecqueur, L.; Duellberg, C.; Dreier, B.; Jiang, Q.; Wang, C.; Pluckthun, A.; Surrey, T.; Gigant, B.; Knossow, M. A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proceedings of the National Academy of Sciences of the U.S.A., 2012, 109(30), 12011-12016. doi: 10.1073/pnas.1204129109
  58. Sparks, J.; Slobodkin, G.; Matar, M.; Congo, R.; Ulkoski, D.; Rea-Ramsey, A.; Pence, C.; Rice, J.; McClure, D.; Polach, K.J. et al. Versatile cationic lipids for siRNA delivery. Journal of Controlled Release, 2012, 158(2), 269-276. doi: 10.1016/j.jconrel.2011.11.006
Show more (54)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.
translate