SynaptoProbe Red

Neuroscience Synaptic vesicles Microscopy Cell membrane
Cat. # Quantity Price Lead time
3425-100ug 100 ug –   in stock
3425-1mg 1 mg $132 in stock
3425-5mg 5 mg $350 in stock
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SynaptoProbe Red is an analog of FM®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.

This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.

SynaptoProbe Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

SynaptoProbe Red, 3425-100ug
SynaptoProbe Red, 3425-100ug
SynaptoProbe Red, 3425-100ug
Structure of SynaptoProbe Red
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Absorption and emission spectra of SynaptoProbe Red

Absorption and emission spectra of SynaptoProbe Red

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General properties

Appearance: dark powder
Molecular weight: 700.51
CAS number: 162112-35-8 (bromide salt)
IUPAC name: 4-{6-[4-(diethylamino)phenyl]hexa-1,3,5-trien-1-yl}-1-[3-(triethylammonio)propyl]pyridinium diiodide
Solubility: good in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 536
Emission maximum, nm: 736
FM® is the trademark of Molecular Probes
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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