F-ara-EdU (2’-Deoxy-2’-fluoro-5-ethynyluridine)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3438-5mg | 5 mg | – | in stock | |
| 3438-25mg | 25 mg | $375 | in stock | |
| 3438-100mg | 100 mg |
$825
|
in stock | |
| 3438-500mg | 500 mg | please inquire | in stock |
F-ara-EdU (2’-deoxy-2’-fluoro-5-ethynyluridine) is a synthetic analog of thymidine used to study de novo DNA synthesis and cell proliferation. It is a less cytotoxic alternative for BrdU (5-Bromo-2’-deoxyuridine) and EdU (5-Ethynyl-2’-deoxyuridine).
F-ara-EdU incorporates into replicating DNA during the S-phase of the cell cycle instead of natural thymidine. Metabolic incorporation of F-ara-EdU into DNA can be detected by copper-catalyzed click reaction with fluorescent or biotin-labeled azides.
In contrast to EdU, F-ara-EdU causes little or no cellular arrest or DNA synthesis inhibition. Therefore, F-ara-EdU is ideally suited for pulse-chase experiments aimed at birth-dating DNA in vivo and long-term cell survival estimation.
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Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.General properties
| Appearance: | off-white powder |
| Molecular weight: | 270.22 |
| CAS number: | 95740-26-4 |
| Molecular formula: | C11H11FN2O5 |
| Solubility: | in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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