Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17020 1 mg 110.00$ in stock
27020 5 mg 210.00$ in stock
47020 25 mg 410.00$ in stock
57020 50 mg 695.00$ in stock
67020 100 mg 1190.00$ in stock

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cy5.5 absorbance and emission spectra

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Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

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Amine reactive NHS ester of near infrared dye Cyanine7.

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

General properties

Appearance: dark blue powder
Molecular weight: 716.31
Molecular formula: C44H46ClN3O4
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 673
ε, L⋅mol−1⋅cm−1: 209000
Emission maximum, nm: 707
Fluorescence quantum yield: 0.2
CF260: 0.11
CF280: 0.08

Product citations

  1. Alam, F.; Chung, S.W.; Hwang, S.R.; Kim, J.-y.; Park, J.; Moon, H.T.; Byun, Y. Preliminary safety evaluation of a taurocholate-conjugated low-molecular-weight heparin derivative (LHT7): a potent angiogenesis inhibitor. Journal of Applied Toxicology, 2015, 35(1), 104–115. doi: 10.1002/jat.2995
  2. Li, L.; Sun, W.; Zhong, J.; Yang, Qi.; Zhu, X.; Zhou, Z.; Zhang Z.; Huang, Y. Multistage Nanovehicle Delivery System Based on Stepwise Size Reduction and Charge Reversal for Programmed Nuclear Targeting of Systemically Administered Anticancer Drugs. Advanced Functional Materials, 2015, 25(26), 4101–4113. doi: 10.1002/adfm.201501248
  3. Zhou, J.; Joshi, B.P.; Duan, X.; Pant, A.; Qiu, Z.; Kuick, R.; Owens, S.R.; Wang, T.D. EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clinical and Translational Gastroenterology, 2015, 6, e101. doi: 10.1038/ctg.2015.28
  4. Arami, H.; Khandhar, A.P.; Tomitaka, A.; Yu, E.; Goodwill, P.W.; Conolly, S.M.; Krishnan, K.M. In vivo multimodal magnetic particle imaging (MPI) with tailored magneto/optical contrast agents. Biomaterials, 2015, 52, 251–261. doi: 10.1016/j.biomaterials.2015.02.040
  5. Mahoney, D.; Owens, E.A.; Fan, C.; Hsiang, J.-C.; Henary, M.; Dickson, R.M. Tailoring Cyanine Dark States for Improved Optically Modulated Fluorescence Recovery. The Journal of Physical Chemistry B, 2015, 119(13), 4637–4643. doi: 10.1021/acs.jpcb.5b00777
  6. Bygd, H.C.; Forsmark, K.D.; Bratlie, K.M. Altering in vivo macrophage responses with modified polymer properties. Biomaterials, 2015, 56, 187–197. doi: 10.1016/j.biomaterials.2015.03.042
  7. Hou, Z.; Lin, J.; Li, Y.; Guo, F.; Yu, F.; Wu, H.; Fan, Z.; Zhi, L.; Luo, F. Validation of a dual role of methotrexate-based chitosan nanoparticles in vivo. RSC Advances, 2015, 5(52), 41393–41400. doi: 10.1039/c5ra03705K
  8. Ruan, S.; He, Q.; Gao, H. Matrix metalloproteinase triggered size-shrinkable gelatin-gold fabricated nanoparticles for tumor microenvironment sensitive penetration and diagnosis of glioma. Nanoscale, 2015, 7, 9487–9496. doi: 10.1039/c5nr01408e
  9. Kim, M.-G.; Park, J.Y.; Miao, W.; Lee, J.; Oh, Y.-K. Polyaptamer DNA nanothread-anchored, reduced graphene oxide nanosheets for targeted delivery. Biomaterials, 2015, 48, 129–136. doi: 10.1016/j.biomaterials.2015.01.009
  10. Cai, H.; Singh, A.N.; Sun, X.; Peng, F. Synthesis and Characterization of Her2-NLP Peptide Conjugates Targeting Circulating Breast Cancer Cells: Cellular Uptake and Localization by Fluorescent Microscopic Imaging. Journal of Fluorescence, 2015, 25(1), 113–117. doi: 10.1007/s10895-014-1486-9
  11. Yang, Q.; Li, L.; Zhu, X.; Sun, W.; Zhou, Z.; Huang, Y. The impact of the HPMA polymer structure on the targeting performance of the conjugated hydrophobic ligand. RSC Advances, 2015, 5(19), 14858–14870. doi: 10.1039/c4ra16085a
  12. Cai, L.; Dewi, R.E.; Heilshorn, S.C. Injectable Hydrogels with In Situ Double Network Formation Enhance Retention of Transplanted Stem Cells. Advanced Functional Materials, 2015, 25(9), 1344–1351. doi: 10.1002/adfm.201403631
  13. Jeong, J.-H. Molecular imaging monitoring of poly(ethylene glycol) conjugated islets for evaluation of islet graft rejection. Archives of Pharmacal Research, 2015, 38(5), 785–790. doi: 10.1007/s12272-014-0412-7
  14. Wang, X.; Huang, S.S.; Heston, W.D.W.; Guo, H.; Wang, B.-C.; Basilion, J.P. Development of Targeted Near-Infrared Imaging Agents for Prostate Cancer. Molecular Cancer Therapeutics, 2014, 13(11), 2595–2606. doi: 10.1158/1535-7163.mct-14-0422
  15. Kim, H.S.; Yoo, H.S. Surface-polymerized biomimetic nanofibrils for the cell-directed association of 3-D scaffolds. Chemical Communications, 2014, 51, 306–309. doi: 10.1039/c4cc06875k
  16. Al-Hilal, T.A.; Alam, F.; Park, J.W.; Kim, K.; Kwon, I.C.; Ryu, G.H.; Byun, Y. Prevention effect of orally active heparin conjugate on cancer-associated thrombosis. Journal of Controlled Release, 2014, 195, 155-161. doi: 10.1016/j.jconrel.2014.05.027
  17. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  18. Hu, S.-H.; Fang, R.-H.; Chen, Y.-W.; Liao, B.-J.; Chen, I.-W.; Chen, S.-Y. Photoresponsive Protein-Graphene-Protein Hybrid Capsules with Dual Targeted Heat-Triggered Drug Delivery Approach for Enhanced Tumor Therapy. Advanced Functional Materials, 2014, 24(26), 4144-4155. doi: 10.1002/adfm.201400080
  19. Lv, S.; Tang, Z.; Li, M.; Lin, J.; Song, W.; Liu, H.; Huang, Y.; Zhang, Y.; Chen, X. Co-delivery of doxorubicin and paclitaxel by PEG-polypeptide nanovehicle for the treatment of non-small cell lung cancer. Biomaterials, 2014, 35(23), 6118-6129. doi: 10.1016/j.biomaterials.2014.04.034
  20. Morton, S.W.; Lee, M.J.; Deng, Z.J.; Dreaden, E.C.; Siouve, E.; Shopsowitz, K.E.; Shah, N.J.; Yaffe, M.B.; Hammond, P.T. A Nanoparticle-Based Combination Chemotherapy Delivery System for Enhanced Tumor Killing by Dynamic Rewiring of Signaling Pathways. Science Signaling, 2014, 7(325), ra44-ra44. doi: 10.1126/scisignal.2005261
  21. Zou, Y.; Li, F.; Hou, W.; Sampath, P.; Zhang, Y.; Thorne, S.H. Manipulating the expression of chemokine receptors enhances delivery and activity of cytokine-induced killer cells. British Journal of Cancer, 2014, 110(8), 1992-1999. doi: 10.1038/bjc.2014.140
  22. Joshi, R.; Feldmann, V.; Koestner, W.; Detje, C.; Gottschalk, S.; Mayer, H.A.; Sauer, M.G.; Engelmann, J. Multifunctional silica nanoparticles for optical and magnetic resonance imaging. Biological Chemistry, 2013, 394(1), 125–135. doi: 10.1515/hsz-2012-0251
  23. Lee, A.; Chin, J.; Park, O.K.; Chung, H.; Kim, J.W.; Yoon, S.-Y.; Park, K. A novel near-infrared fluorescence chemosensor for copper ion detection using click ligation and energy transfer. Chemical Communications, 2013, 49, 5969–5971. doi: 10.1039/c3cc42059k
  24. Morton, S.W.; Herlihy, K.P.; Shopsowitz, K.E.; Deng, Z.J.; Chu, K.S.; Bowerman, C.J.; DeSimone, J.M.; Hammond, P.T. Scalable Manufacture of Built-to-Order Nanomedicine: Spray-Assisted Layer-by-Layer Functionalization of PRINT Nanoparticles. Advanced Materials, 2013, 25(34), 4707-4713. doi: 10.1002/adma.201302025
  25. Tang, H.; Sampath, P.; Yan, X.; Thorne, S.H. Potential for enhanced therapeutic activity of biological cancer therapies with doxycycline combination. Gene Therapy, 2013, 20(7), 770-778. doi: 10.1038/gt.2012.96
  26. Wang, C.; Ravi, S.; Garapati, U.S.; Das, M.; Howell, M.; Mallela, J.; Alwarappan, S.; Mohapatra, S.S.; Mohapatra, S. Multifunctional chitosan magnetic-graphene (CMG) nanoparticles: a theranostic platform for tumor-targeted co-delivery of drugs, genes and MRI contrast agents. Journal of Materials Chemistry B, 2013, 1(35), 4396-4396. doi: 10.1039/c3tb20452a
  27. Yuan, H.; Cho, H.; Chen, H.H.; Panagia, M.; Sosnovik, D.E.; Josephson, L. Fluorescent and radiolabeled triphenylphosphonium probes for imaging mitochondria. Chemical Communications, 2013, 49(88), 10361-10363. doi: 10.1039/c3cc45802d
  28. Liu, Z.; Miller, S.J.; Joshi, B.P.; Wang, T.D. In vivo targeting of colonic dysplasia on fluorescence endoscopy with near-infrared octapeptide. Gut, 2012, 62(3), 395-403. doi: 10.1136/gutjnl-2011-301913
  29. Adulnirath, A.; Chung, S.W.; Park, J.; Hwang, S.R.; Kim, J.-Y.; Yang, V.C.; Kim, S.Y.; Moon, H.T.; Byun, Y. Cyclic RGDyk-conjugated LMWH-taurocholate derivative as a targeting angiogenesis inhibitor. Journal of Controlled Release, 2012, 164(1), 8-16. doi: 10.1016/j.jconrel.2012.10.001
  30. Kim, J.-y.; Shim, G.; Choi, H.-w.; Park, J.; Chung, S.W.; Kim, S.; Kim, K.; Chan Kwon, I.; Kim, C.-W.; Kim, S.Y. et al. Tumor vasculature targeting following co-delivery of heparin-taurocholate conjugate and suberoylanilide hydroxamic acid using cationic nanolipoplex. Biomaterials, 2012, 33(17), 4424-4430. doi: 10.1016/j.biomaterials.2012.02.066
  31. Zhou, K.; Liu, H.; Zhang, S.; Huang, X.; Wang, Y.; Huang, G.; Sumer, B.D.; Gao, J. Multicolored pH-Tunable and Activatable Fluorescence Nanoplatform Responsive to Physiologic pH Stimuli. Journal of the American Chemical Society, 2012, 134(18), 7803-7811. doi: 10.1021/ja300176w
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