Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17020 1 mg $125.00 in stock
27020 5 mg $260.00 in stock
47020 25 mg $510.00 in stock
57020 50 mg $895.00 in stock
67020 100 mg $1490.00 in stock

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.

Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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General properties

Appearance: dark blue to violet solid
Molecular weight: 767.66
CAS number: 2375105-86-3
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Su, D.; Tsai, H.-I.; Xu, Z.; Yan, F.; Wu, Y.; Xiao, Y.; Liu, X.; Wu, Y.; Parvanian, S.; Zhu, W.; Eriksson, J.E.; Wang, D.; Zhu, H.; Chen, H.; Cheng, F. Exosomal PD-L1 functions as an immunosuppressant to promote wound healing. Journal of Extracellular Vesicles, 2024, 9(1), 1709262. doi: 10.1080/20013078.2019.1709262
  2. Han, G.; Kim, H.; Jang, H.; Kim, E.S.; Kim, S.H.; Yang, Y. Oral TNF-α siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease. Bioactive Materials, 2024, 34, 138-149. doi: 10.1016/j.bioactmat.2023.12.010
  3. Okamoto, W.; Usui, T.; Hasegawa, M.; Kobayashi, T.; Fujisawa, J.; Taguchi, K.; Matsumoto, K.; Kohno, M.; Iwazaki, M.; Shimano, S.; Nagao, I.; Toyoda, H.; Matsumura, N.; Tomiyasu, H.; Tochinai, R.; Komatsu, T. Polyoxazoline-Conjugated Porcine Serum Albumin as an Artificial Plasma Expander for Dogs. Sci Rep, 2023, 13(1), 9512. doi: 10.1038/s41598-023-35999-4
  4. Yao, P.; Liu, R.; Broginni, T.; Thunemann, M.; Kleinfeld, D. Guide to the Construction and Use of an Adaptive Optics Two-Photon Microscope with Direct Wavefront Sensing. bioRxiv, 2023, preprint. doi: 10.1101/2023.01.24.525307
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