SIMA azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| AE230 | 1 mg | $110 | in stock | |
| BE230 | 5 mg | $180 | in stock | |
| CE230 | 10 mg | $310 | in stock | |
| DE230 | 25 mg |
$410
|
in stock | |
| EE230 | 50 mg |
$695
|
in stock | |
| FE230 | 100 mg |
$1190
|
in stock |
SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.
SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.
Absorption and emission spectra of SIMA
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Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.SIMA phosphoramidite, 6-isomer (hydroxyprolinol)
A phosphoramidite (hydroxyprolinol) for the synthesis of SIMA-labeled oligonucleotides. SIMA is a more stable analog of HEX.General properties
| Appearance: | orange powder |
| Molecular weight: | 679.52 |
| Molecular formula: | C36H24Cl2N4O6 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 531 |
| ε, L⋅mol−1⋅cm−1: | 92300 |
| Emission maximum, nm: | 555 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.57 |
| CF280: | 0.18 |
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