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FAM azide, 5- isomer, 10 mM solution in DMSO
| Lot No | Quantity | Price | |
|---|---|---|---|
| 14130 | 100 uL | $ 160.00 | |
| 34130 | 500 uL | $ 420.00 | |
| 44130 | 1 mL | $ 595.00 |
FAM (fluorescein) azide, 10 mM solution in DMSO, labeling reagent for Click Chemistry.
Pure 5-isomer.
Can replace Alexa Fluor 488, DyLight 488.
| General properties | |
|---|---|
| Appearance: | yellowish solution1 |
| Molar weight: | 458.42 |
| Mass spec M+ increase: | 458.4 |
| Spectral properties | |
| FAM excitation maximum, λmaxex, nm: | 494 |
| FAM extinction at λmaxex | 75,000 |
| FAM emission maximum, λmaxfluor, nm: | 520 |
| FAM fluorescence quantum yield: | 0.9 |
| Quality control | |
| NMR: | 95+% |
| TLC: | pure |
| Functional testing (CuAAC): | pass |
| Recommended protocols | |
| Click-chemistry labeling of oligonucleotides and DNA | HTML | PDF |
| Storage conditions | |
| Store at -20°C in the dark. | |
1 Fluorescein is non-fluorescent and very slightly colored in DMSO because it forms cyclic form (structure shown above). When diluted with water, the solution becomes brightly green-fluorescent orange.
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