DAPI, blue fluorescent nucleic acid stain
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2996-1mL | 1 mL, 1 mg/mL in Water | $75 | in stock | |
2996-50uL | 50 uL, 1 mg/mL in Water | – | in stock | |
2996-1mg | 1 mg | $30 | in stock | |
2996-10mg | 10 mg | $110 | in stock |
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.
When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 358 nm and emission maximum at 461 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.
There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.
Absorption and emission spectra of DAPI
Customers also purchased with this product
Hoechst 33342, blue fluorescent nucleic acid stain
Hoechst 33342 (bisbenzimide) is a cell-permeable blue-emitting fluorescent dsDNA dye used for staining chromosomes and nuclei in live and fixed cells.sulfo-Cyanine5 NHS ester
Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.Cyanine7 NHS ester
Amine reactive NHS ester of near infrared dye Cyanine7.get free express delivery
General properties
Appearance: | yellow solid / solution |
Molecular weight: | 350.25 |
CAS number: | 28718-90-3 |
Molecular formula: | C16H17Cl2N5 |
Solubility: | good in DMF, DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 355 |
ε, L⋅mol−1⋅cm−1: | 28800 |
Emission maximum, nm: | 460 |
Product citations
- Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413