Cyanine5 phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1740-100mg | 100 mg | – | in stock | |
| 1740-250mg | 250 mg | $290 | in stock | |
| 1740-1g | 1 g |
$950
|
in stock | |
| 1740-10g | 10 g |
$6750
|
7 days |
Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of the fluorophore. This is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.
Absorption and emission spectra of Cyanine5
Customers also purchased with this product
FAM phosphoramidite, 6-isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.TFA-amino modifier CPG 500
TFA-amino modifier CPG for oligo synthesis. 500 Å, high load controlled pore glass.ROX azide, 5-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 816.49 |
| Molecular formula: | C47H67N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
The count of items is incorrect.
Your cart is empty
$ 
