BDP 558/568 DBCO

Cat. # Quantity Price Lead time
174F0 1 mg $125 in stock
274F0 5 mg $260 in stock
374F0 10 mg $325 in stock
474F0 25 mg $510 in stock
574F0 50 mg $895 in stock
674F0 100 mg $1490 in stock
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BDP 558/568 fluorescence maximum is in the yellow spectrum range (569 nm). This dye is an analog of such fluorophore as Cy3™ by its spectral characteristics. It is soluble in polar organic solvents and has good photostability and high quantum yield.

BDP 558/568 has hydrophobic properties and can be used for staining lipids, membranes, and other lipophilic compounds.

Introduced in the BDP 558/568 molecule, the cyclooctyne functional group allows entering into reactions of 1,3-dipolar cycloaddition with various substituted azides. Such reactions are promoted by ring strain and do not require copper catalysis. Thus, this method can be used for investigating various processes in living cells.

BDP 558/568 DBCO can be used for the detection of target molecules, proteins, or nucleic acids that contain azide groups by microscopy and flow cytometry.

Absorption and emission spectra of BDP 558/568

Absorption and emission spectra of BDP 558/568

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DBCO NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.

BDP 558/568 tetrazine

Stable fluorophore that can be conjugated with trans-cyclooctene derivatives of biomolecules for their visualization.

Cyanine5 tetrazine

Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.

General properties

Appearance: brown powder with luster
Molecular weight: 646.56
Molecular formula: C37H33N4BF2O2S
Solubility: very soluble in DMF, DMSO, dichloromethane
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 561
ε, L⋅mol−1⋅cm−1: 84400
Emission maximum, nm: 569
Fluorescence quantum yield: 0.68
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