TET azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 18230 | 1 mg | $110 | in stock | |
| 28230 | 5 mg | $165 | in stock | |
| 48230 | 25 mg | $285 | in stock | |
| 58230 | 50 mg |
$495
|
in stock | |
| 68230 | 100 mg |
$880
|
in stock |
TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate with absorption maximum at 519 nm and emission maximum at 535 nm. TET is spectrally similar to R6G, JOE, and VIC, widely used for labeling PCR probes.
Oligonucleotides labeled with TET are often used in real-time PCR; the preparation of such oligonucleotides can be performed using click chemistry. This derivative is an azide, a pure 6-isomer, for conjugating TET to other molecules by copper-catalyzed and copper-free click reactions.
Absorption and emission spectra of TET
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JOE azide, 5-isomer
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VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.R6G azide, 5-isomer
Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.General properties
| Appearance: | orange powder |
| Molecular weight: | 596.21 |
| CAS number: | 1450752-90-5 |
| Molecular formula: | C24H14Cl4N4O6 |
| Solubility: | in DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 100000 |
| Emission maximum, nm: | 535 |
| Fluorescence quantum yield: | 0.47 |
| CF260: | 0.17 |
| CF280: | 0.09 |
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