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Sulfo-Cy3 NHS ester
| Lot No | Quantity | Price | |
|---|---|---|---|
| 11320 | 1 mg | $ 160.00 | |
| 21320 | 5 mg | $ 410.00 | |
| 41320 | 25 mg | $ 850.00 | |
| 51320 | 50 mg | $ 1500.00 | |
| 61320 | 100 mg | $ 2450.00 |
Water soluble, amino-reactive Cy3 dye. Efficiently labels proteins and peptides in aqueous solution, without need to add organic co-solvent.
This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cy3 NHS ester is also available.
Cy3 NHS ester replaces Alexa Fluor 546, and DyLight 549 for all applications.
| General properties | |
|---|---|
| Appearance: | dark red powder |
| Solubility: | Good in water and polar organic solvents (DMF, DMSO), moderate in alcohols and acetonitrile, poor in organic solvents (DCM, chloroform) |
| Molar weight: | 735.80 |
| Mass spec M+ increase: | 598.2 |
| Spectral properties | |
| Cy3 excitation maximum, λmaxex, nm: | 546 |
| Cy3 extinction at λmaxex | 150,000 |
| Cy3 emission maximum, λmaxfluor, nm: | 563 |
| Cy3 fluorescence quantum yield: | 0.1 |
| Quality control | |
| NMR: | 95+% |
| TLC: | pure |
| Functional testing (reaction with amine): | pass |
| Recommended protocols | |
| NHS ester labeling of amino biomolecules | HTML | PDF |
| Storage conditions | |
| Store NHS esters at -20°C in the dark. Desiccate. | |
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