Cyanine3 NHS ester

Cat. # Quantity Price Lead time Buy this product
11020 1 mg 110.00$ in stock
21020 5 mg 210.00$ in stock
41020 25 mg 410.00$ in stock
51020 50 mg 695.00$ in stock
61020 100 mg 1190.00$ 5 days

Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.

Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.

Cy3 absorbance and emission spectra

General properties

Appearance: red powder
Mass spec M+ increment: 474.2
Molecular weight: 590.15
Molecular formula: C34H40ClN3O4
CAS number: 1032678-38-8, 1032815-92-1
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31

Product citations

  1. Choi, E.B.; Choi, J.; Bae, S.R.; Kim, H.-O.; Jang, E.; Kang, B.; Kim, M.-H.; Kim, B.; Suh, J.-S.; Lee, K. et al. Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport. Nano Research, in press. doi: 10.1007/s12274-014-0597-6
  2. Rammohan, J.; Ruiz Manzano, A.; Garner, A.L.; Stallings, C.L.; Galburt, E.A. CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism. Nucleic Acids Research, 2015, 43(6), 3272–3285. doi: 10.1093/nar/gkv078
  3. Baker, Matthew B. and Albertazzi, Lorenzo and Voets, Ilja K. and Leenders, Christianus M.A. and Palmans, Anja R.A. and Pavan, Giovanni M. and Meijer, E.W. Consequences of chirality on the dynamics of a water-soluble supramolecular polymer. Nature Communications, 2015, 6, 6234. doi: 10.1038/ncomms7234
  4. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  5. Kim, J.; Yang, Y.; Song, S.S.; Na, J.-H.; Oh, K.J.; Jeong, C.; Yu, Y.G.; Shin, Y.-K. Beta-Amyloid Oligomers Activate Apoptotic BAK Pore for Cytochrome c Release. Biophysical Journal, 2014, 107(7), 1601-1608. doi: 10.1016/j.bpj.2014.07.074
  6. Petkau-Milroy, K.; Sonntag, M.; Colditz, A.; Brunsveld, L. Multivalent Protein Assembly Using Monovalent Self-Assembling Building Blocks. International Journal of Molecular Sciences, 2013, 14(10), 21189–21201. doi: 10.3390/ijms141021189
  7. Albertazzi, L.; Martinez-Veracoechea, F.J.; Leenders, C.M.A.; Voets, I.K.; Frenkel, D.; Meijer, E.W. Spatiotemporal control and superselectivity in supramolecular polymers using multivalency. Proceedings of the National Academy of Sciences, 2013, 110(30), 12203-12208. doi: 10.1073/pnas.1303109110
  8. Cao, Z.; Partyka, K.; McDonald, M.; Brouhard, E.; Hincapie, M.; Brand, R.E.; Hancock, W.S.; Haab, B.B. Modulation of Glycan Detection on Specific Glycoproteins by Lectin Multimerization. Analytical Chemistry, 2013, 85(3), 1689-1698. doi: 10.1021/ac302826a
  9. Haller, A.; Altman, R.B.; Souliere, M.F.; Blanchard, S.C.; Micura, R. Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution. Proceedings of the National Academy of Sciences, 2013, 110(11), 4188-4193. doi: 10.1073/pnas.1218062110
  10. Kaastrup, K.; Chan, L.; Sikes, H.D. Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions. Analytical Chemistry, 2013, 85(17), 8055-8060. doi: 10.1021/ac4018988
  11. Gatzogiannis, E.; Chen, Z.; Wei, L.; Wombacher, R.; Kao, Y.-T.; Yefremov, G.; Cornish, V.W.; Min, W. Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag. Chemical Communications, 2012, 48(69), 8694-8694. doi: 10.1039/c2cc33133k
  12. Kaastrup, K.; Sikes, H.D. Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times. Lab on a Chip, 2012, 12(20), 4055-4055. doi: 10.1039/c2lc40584a
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