Copper(II)-THPTA catalytic buffer, 1.5x
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
B5150 | 1 mL | $30 | in stock | |
K5150 | 10 mL | $120 | in stock | |
S5150 | 50 mL | $490 | in stock |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
Recommended protocol
Calculator
Customers also purchased with this product
Alkyne CPG 1000A
Controlled pore glass solid support for DNA/RNA synthesis with alkyne modification (pore size 1000 Å) for the synthesis of long modified oligonucleotides with 3'-alkyne modification.ROX azide, 5-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.sulfo-Cyanine7.5 azide
sulfo-Cyanine7.5 is a very hydrophilic and water-soluble dye for the near-infrared region. The absorption and emission spectra of the dye are similar to Indocyanine Green (ICG), but sulfo-Cyanine7.5 has a much higher fluorescence quantum yield. The dye is a great choice for near-infrared in vivo imaging. This is an azide for click chemistry.General properties
Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
MSDS: | Download |
Product specifications |