Copper(II)-THPTA catalytic buffer, 1.5x
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| B5150 | 1 mL | $30 | in stock | |
| K5150 | 10 mL | $120 | in stock | |
| S5150 | 50 mL |
$490
|
in stock |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
Recommended protocol
Calculator
Customers also purchased with this product
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester for the labeling of amino-groups.VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.Biotin (hydroxyprolinol) CPG 1000
Solid-phase support based on hydroxyprolinol for the synthesis of oligonucleotides containing biotin at the 3' terminus.General properties
| Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | Download |
| Product specifications |
Your cart is empty
$ 
