AF 430 amine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 108C0 | 1 mg | $125 | in stock | |
| 208C0 | 5 mg | $325 | in stock | |
| 408C0 | 25 mg |
$950
|
in stock | |
| 508C0 | 50 mg |
$1625
|
in stock | |
| 608C0 | 100 mg |
$2090
|
in stock |
AF 430 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the yellow-green region (at 542 nm).
Conjugates of biomolecules with AF 430 can be used in flow cytometry, for example in multicolor staining. AF 430 is also used in cell microscopy due to its high photostability.
AF 430 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.
Absorption and emission spectra of AF 430
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Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.General properties
| Appearance: | yellow solid |
| Molecular weight: | 601.68 |
| Molecular formula: | C28H38N3F3O6S |
| IUPAC name: | (9-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-8,8-dimethyl-2-oxo-4-(trifluoromethyl)-8,9-dihydro-2H-pyrano[3,2-g]quinolin-6-yl)methanesulfonate |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
| CF260: | 0.06 |
| CF280: | 0.06 |
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