SYBR Green I for Real Time PCR, 100x
SYBR Green I is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows to use SYBR Green I as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with SYBR Green I - unlabeled primers are enough.
Unlike other preparations of SYBR Green provided by Lumiprobe and other suppliers for gel staining purposes, this formulation is specially designed to be used for Real Time PCR. Specific features are:
- Concentration of the dye remains constant and optimal from preparation to preparation - the results are reproducible over time
- PCR tested preparation - quality guaranteed
- Low fluorescent background - high fluorescence intensity gain
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
|Appearance:||light orange solution|
|Quality control:||NMR 1H (95%) and 13C, TLC, PCR testing|
|Storage conditions:||Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.|
|Excitation maximum, nm:||454|
|Extinction coefficient at excitation maximum, Lmol-1cm-1:||73000|
|Emission maximum, nm:||524|
|Fluorescence quantum yield:||0,8|
- Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J. Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community. Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795