dsGreen for Real-Time PCR, 100×
|Cat. #||Quantity||Price||Lead time||Buy this product|
|41010||1 mL||310.00$||in stock|
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
SYBR is a registered trademark of Molecular Probes.
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|Appearance:||light orange solution|
|Quality control:||NMR 1H, HPLC-MS (95%), PCR testing|
|Storage conditions:||Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.|
|Excitation maximum, nm:||454|
|Emission maximum, nm:||524|
|Fluorescence quantum yield:||0,8|
- Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J. Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community. Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795