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SYBR Green I for Real Time PCR, 100x

SYBR Green I is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows to use SYBR Green I as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with SYBR Green I - unlabeled primers are enough.

Unlike other preparations of SYBR Green provided by Lumiprobe and other suppliers for gel staining purposes, this formulation is specially designed to be used for Real Time PCR. Specific features are:

  • Concentration of the dye remains constant and optimal from preparation to preparation - the results are reproducible over time
  • PCR tested preparation - quality guaranteed
  • Low fluorescent background - high fluorescence intensity gain

Recommended protocol

Realtime qPCR with SYBR Green I

Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 454
Extinction coefficient at excitation maximum, Lmol-1cm-1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J. Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community. Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795
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