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SYBR Green I for Real Time PCR, 100x
| Lot No | Quantity | Price | |
|---|---|---|---|
| 41010 | 1 mL | $ 310.00 | |
| 51010 | 1.5 mL | $ 450.00 | |
| 51010-5 | 5x1.5 mL | $ 750.00 |
SYBR Green I is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows to use SYBR Green I as a universal dsDNA detection reagent for Real Time PCR. You do not need any labeled probes to detect amplification with SYBR Green I, just usual unlabeled primers.
Unlike other preparations of SYBR Green provided by Lumiprobe and other suppliers for gel staining purposes, this formulation is specially designed to be used for Real Time PCR. Specific features are:
- Concentration of the dye remains constant and optimal from preparation to preparation - the results are reproducible over time
- PCR tested preparation - quality guaranteed
- Low fluorescent background - high fluorescence intensity gain
- Special additives to decrease enzyme inhibition
Protocols are available: View protocol | Download as pdf
Spectral properties
| excitation maximum, λmaxex, nm: | 454 |
| extinction at λmaxex | 73,000 (TE, pH 7.5) |
| fluorescence maximum, λmaxfluor, nm: | 524 |
| fluorescence quantum yield: | 0.8 |
[1]Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
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