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Black Hole 1 (BHQ-1) quencher CPG
| Lot No | Quantity | Price | |
|---|---|---|---|
| 11860 | 100 mg | $ 160.00 | |
| 21860 | 250 mg | $ 350.00 | |
| 31860 | 500 mg | $ 540.00 | |
| 41860 | 1 g | $ 900.00 |
BHQ-1 (Black Hole Quencher 1) CPG is a controlled pore glass solid support for 3'-modification of oligonucleotide. Primary application is synthesis of TaqMan and molecular beacon probes for qPCR.
BHQ-1 is ideal quencher for qPCR probes containing FAM, R110, R6G, and JOE dyes.
1000 Å pore size makes this support useful for the synthesis of oligonucleotides of up to 100 mer.
This product is compatible with oligo synthesis kits provided by Luimprobe.
| General properties | |
|---|---|
| Appearance: | dark brown beads |
| CPG pore size: | 1000 Å |
| Oligonucleotide synthesis conditions | |
| Synthesizer compatibility | all synthesizers using phosphoramidite chemistry |
| Coupling time | standard |
| Deblock conditions | standard (6h in aq. ammonia at 60°C) |
| Oligo purification | HPLC or PAAG |
| Quality control | |
| Trityl loading: | 50-70 umol / g (exact loading specified for each batch) |
| NMR of precursor (1H): | 95+% |
| Storage conditions | |
| Store at -20°C in the dark. Desiccate. | |
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